Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University , Box 332, Shenyang 110819, China.
ACS Appl Mater Interfaces. 2017 Aug 30;9(34):28273-28280. doi: 10.1021/acsami.7b08942. Epub 2017 Aug 16.
Novel unilamellar and homogeneous titanate nanosheets were prepared by anchoring (3-aminopropyl)triethoxysilane (APTES) and chelating copper ions, also know by the short form Cu-APTES-TiNSs. The nanosheets were uniform two-dimensional lamellas/monolayers with a thickness of 1.9 nm, and they were further characterized by atomic force microscopy, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectra, X-ray diffraction, X-ray photoelectron spectroscopy, inductively coupled plasma mass spectrometry, and N adsorption-desorption. The copper-decorated titanate nanosheets possess a copper content of 4.28 ± 0.14% and exhibit a favorable selectivity to the adsorption of hemoglobin, with a considerable capacity of 5314.2 mg g. The adsorbed hemoglobin is easily collected with a recovery rate of 91.3% by using 0.5% w/v sodium dodecyl sulfate as an eluent. Circular dichroism spectra confirmed that virtually no conformational alteration is observed for hemoglobin. Cu-APTES-TiNSs are further applied for the selective adsorption of hemoglobin from the human whole blood.
新型单层且均匀的钛酸钠纳米片是通过锚定（3-氨丙基）三乙氧基硅烷（APTES）和螯合铜离子制备的，也称为 Cu-APTES-TiNSs。这些纳米片为均匀的二维薄片/单层，厚度为 1.9nm，其进一步通过原子力显微镜、扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱、X 射线衍射、X 射线光电子能谱、电感耦合等离子体质谱和 N2 吸附-脱附进行了表征。铜修饰的钛酸钠纳米片具有 4.28±0.14%的铜含量，对血红蛋白的吸附表现出良好的选择性，吸附容量高达 5314.2mg/g。血红蛋白的回收率为 91.3%，使用 0.5%w/v 十二烷基硫酸钠作为洗脱液，很容易收集到吸附的血红蛋白。圆二色谱证实血红蛋白几乎没有发生构象变化。Cu-APTES-TiNSs 进一步用于从人全血中选择性吸附血红蛋白。
