LINC00152 的 lncRNA 敲低通过 EGFR/PI3K/AKT 通路对肺癌的生物学活性具有抑制作用。
lncRNA LINC00152 knockdown had effects to suppress biological activity of lung cancer via EGFR/PI3K/AKT pathway.
机构信息
Tumor Four Departments, People's Hospital of Hebei Medical University, People's Republic of China.
Tumor Four Departments, People's Hospital of Hebei Medical University, People's Republic of China.
出版信息
Biomed Pharmacother. 2017 Oct;94:644-651. doi: 10.1016/j.biopha.2017.07.120. Epub 2017 Aug 5.
Accumulating evidence demonstrates that lncRNAs play important roles in regulating gene expression and are involved in various pathological processes. In our present study, we firstly evaluated lncRNA LINC00152 and EGFR expressions by ISH or IHC methods, and analyzed the correlation between LINC00152 and EGFR with RT-PCR. lncRNA LINC00152 of NSCLC tissues were significantly up-regulation compared with adjacent normal tissues and positively correlated with EGPR. The further cell experiments demonstrated that Linc00152 knockdown had effects of suppression cell proliferation, invasion and migration abilities and improving cell apoptosis and G1 phase rates in both A549 and H1299 cell lines. In the mechanism study, the results were shown that EGFR, PI3K, AKT, Fibronectin and Vimentin proteins expressions were significantly reduced and P21 protein expression was significantly increased in Linc00152 knockdown groups. Our results suggested lncRNA LINC00152 knock-down had anti-tumor effects via EGFR/PI3K/AKT pathway.
越来越多的证据表明，lncRNAs 在调节基因表达方面发挥着重要作用，并参与各种病理过程。在本研究中，我们首先通过ISH 或 IHC 方法评估了 lncRNA LINC00152 和 EGFR 的表达，并通过 RT-PCR 分析了 LINC00152 与 EGFR 之间的相关性。与相邻正常组织相比，非小细胞肺癌组织中的 lncRNA LINC00152 明显上调，并与 EGPR 呈正相关。进一步的细胞实验表明，在 A549 和 H1299 细胞系中，Linc00152 敲低可抑制细胞增殖、侵袭和迁移能力，并提高细胞凋亡和 G1 期比率。在机制研究中，结果表明，在 Linc00152 敲低组中，EGFR、PI3K、AKT、纤连蛋白和波形蛋白蛋白表达显著降低，P21 蛋白表达显著增加。我们的研究结果表明，lncRNA LINC00152 通过 EGFR/PI3K/AKT 通路发挥抗肿瘤作用。
Zotero 插件