长链非编码 RNA MEG3 具有抑制宫颈癌的活性。
LncRNA MEG3 has anti-activity effects of cervical cancer.
机构信息
Department of Gynaecology, Yantaishan Hospital, Yantai City, Shandong Province, 264000, China.
Department of Digestology, Yuhuangding Hospital, Yantai City, Shandong Province, 264000, China.
出版信息
Biomed Pharmacother. 2017 Oct;94:636-643. doi: 10.1016/j.biopha.2017.07.056. Epub 2017 Aug 5.
AIM
The aim of this study was to explain the lncRNA MEG3 had anti-cancer effects to suppress cervical carcinoma biological activity.
METHODS
The Hela cell were divided into three groups (NC group,BL group and lncRNA group). The cells of lncRNA or BL groups were transfered with lncRNA MEG3 or blank carrier. Evaluating the cell proliferation rate of difference groups by MTT assay; measuring the cell apoptosis and cell cycle of difference groups' cell by flow-cytometry; the cell invasion activity of difference groups were measured by transwell assay, the cell migration ability of difference groups were evaluated by wound healing testing. Measuring the relative gene expressions (PI3K, AKT, MMP-2, MMP-9, Bcl-2, Bax and P21) and protein expressions (PI3K, AKT, p-AKT, MMP-2, MMP-9, Bcl-2, Bax and P21) by RT-PCR or WB assay.
RESULTS
Compared with NC group, The cell proliferation rate of lncRNA group was significantly reduced (P<0.05) and the cell apoptosis and G1 phase were significantly increased (P<0.05, respectively). The invasion cell of lncRNA MEG3 group were significantly difference compared with NC group (P<0.05), and the wound healing rate of lncRNA MEG3 group was significantly shorter than NC group (P<0.05). The PI3K, AKT, MMP-2, MMP-9 and Bcl-2 gene expression of lncRNA group were significanlty down-regulation compared with NC group (P<0.05,respectively), and Bax and P21 gene expression of lncRNA group were significantly up-regulation compared with NC group (P<0.05,respectively) by RT-PCR testing. The PI3K, AKT, p-AKT, MMP-2, MMP-9 and Bcl-2 protein expression of lncRNA group were significanlty down-regulation compared with NC group (P<0.05,respectively), and Bax and P21 protein expression of lncRNA group were significantly up-regulation compared with NC group (P<0.05,respectively) by WB assay.
CONCLUSION
The lncRNA MEG3 had effects to supress cervical cancer by regulation PI3K/AKT/Bcl-2/Bax/P21 and PI3K/AKT/MMP-2/9 signaling pathway.
目的
本研究旨在解释长链非编码 RNA MEG3 具有抑制宫颈癌生物活性的抗癌作用。
方法
将 Hela 细胞分为三组（NC 组、BL 组和 lncRNA 组）。lncRNA 或 BL 组的细胞转染 lncRNA MEG3 或空白载体。通过 MTT 测定评估不同组的细胞增殖率；通过流式细胞术测量不同组细胞的细胞凋亡和细胞周期；通过 Transwell 测定测量不同组的细胞侵袭活性，通过划痕愈合试验评估不同组的细胞迁移能力。通过 RT-PCR 或 WB 测定测量相对基因表达（PI3K、AKT、MMP-2、MMP-9、Bcl-2、Bax 和 P21）和蛋白表达（PI3K、AKT、p-AKT、MMP-2、MMP-9、Bcl-2、Bax 和 P21）。
结果
与 NC 组相比，lncRNA 组的细胞增殖率显著降低（P<0.05），细胞凋亡和 G1 期显著增加（P<0.05，分别）。lncRNA MEG3 组的侵袭细胞与 NC 组相比差异有统计学意义（P<0.05），lncRNA MEG3 组的伤口愈合率明显短于 NC 组（P<0.05）。lncRNA 组的 PI3K、AKT、MMP-2、MMP-9 和 Bcl-2 基因表达与 NC 组相比显著下调（P<0.05，分别），lncRNA 组的 Bax 和 P21 基因表达与 NC 组相比显著上调（P<0.05，分别）通过 RT-PCR 检测。lncRNA 组的 PI3K、AKT、p-AKT、MMP-2、MMP-9 和 Bcl-2 蛋白表达与 NC 组相比显著下调（P<0.05，分别），lncRNA 组的 Bax 和 P21 蛋白表达与 NC 组相比显著上调（P<0.05，分别）通过 WB 检测。
结论
lncRNA MEG3 通过调节 PI3K/AKT/Bcl-2/Bax/P21 和 PI3K/AKT/MMP-2/9 信号通路抑制宫颈癌。
Zotero 插件