Jonitz-Heincke Anika, Wieding Jan, Schulze Christoph, Hansmann Doris, Bader Rainer
Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medicine Rostock, Doberaner Strasse 142, Rostock 18057, Germany.
Department of Orthopaedic Surgery, Armed Forces Hospital Westerstede, Lange Str. 38, Westerstede 26655, Germany.
Materials (Basel). 2013 Nov 21;6(11):5398-5409. doi: 10.3390/ma6115398.
Synthetic materials for bone replacement must ensure a sufficient mechanical stability and an adequate cell proliferation within the structures. Hereby, titanium materials are suitable for producing patient-individual porous bone scaffolds by using generative techniques. In this study, the viability of human osteoblasts was investigated in porous 3D Ti6Al4V scaffolds, which were produced by electron-beam (EBM) or laser-beam melting (LBM). For each examination, two cylindrical scaffolds (30 mm × 10 mm in size, 700 µm × 700 µm macropores) were placed on each other and seeded with cells. The oxygen consumption and the acidification in the center of the structures were investigated by means of microsensors. Additionally, the synthesis of pro-collagen type 1 was analyzed. On the LBM titanium scaffolds, vital bone cells were detected in the center and in the periphery after 8 days of cultivation. In the EBM titanium constructs, however, vital cells were only visible in the center. During the cultivation period, the cells increasingly produced procollagen type 1 in both scaffolds. In comparison to the periphery, the oxygen content in the center of the scaffolds slightly decreased. Furthermore, a slight acidification of the medium was detectable. Compared to LBM, the EBM titanium scaffolds showed a less favorable behavior with regard to cell seeding.
用于骨替代的合成材料必须确保结构内有足够的机械稳定性和充足的细胞增殖。因此，钛材料适合通过生成技术制造患者个体化的多孔骨支架。在本研究中，研究了人成骨细胞在通过电子束熔炼（EBM）或激光束熔炼（LBM）制造的多孔三维Ti6Al4V支架中的活力。每次检测时，将两个圆柱形支架（尺寸为30 mm×10 mm，大孔为700 µm×700 µm）相互叠放并接种细胞。通过微传感器研究结构中心的氧气消耗和酸化情况。此外，还分析了I型前胶原的合成。在培养8天后，在LBM钛支架的中心和周边均检测到有活力的骨细胞。然而，在EBM钛结构中，仅在中心可见有活力的细胞。在培养期间，两种支架中的细胞均越来越多地产生I型前胶原。与周边相比，支架中心的氧含量略有下降。此外，可检测到培养基有轻微酸化。与LBM相比，EBM钛支架在细胞接种方面表现出较不利的情况。
