Liu Lingbin, Wang Guoqing, Xiao Yang, Shipp Steven L, Siegel Paul B, Cline Mark A, Gilbert Elizabeth R
Sichuan Agricultural University, Ya'an 625014, Sichuan, China.
Virginia Polytechnic Institute and State University, Department of Animal and Poultry Sciences, Blacksburg, Virginia 24061, United States.
Comp Biochem Physiol A Mol Integr Physiol. 2017 Nov;213:1-10. doi: 10.1016/j.cbpa.2017.08.001. Epub 2017 Aug 5.
Neuropeptide Y (NPY) stimulates appetite and promotes lipid deposition. We demonstrated a differential sensitivity in the food intake response to central NPY in chicks from lines selected for low (LWS) or high (HWS) body weight, but have not reported whether such differences exist in the periphery. At 5days, LWS and HWS chicks were intraperitoneally injected with 0 (vehicle), 60, or 120μg/kg BW NPY and subcutaneous adipose tissue and plasma were collected at 1, 3, 6, 12, and 24h (n=12). NPY injection increased glycerol-3-phosphate dehydrogenase (G3PDH) activity at 1 and 3h and reduced plasma non-esterified fatty acids (NEFAs) at 1 and 12h. G3PDH activity was greater in HWS than LWS while NEFAs were greater in LWS. At 1h, peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding protein (C/EBP)α, and microsomal triglyceride transfer protein (MTTP) mRNAs were reduced in NPY-injected chicks whereas NPY receptor 1 (NPYR1) was increased. Expression of stearoyl-CoA desaturase (SCD1) was increased by NPY at 1h in HWS but not LWS. PPARγ (3 and 6h), C/EBPβ (3h), C/EBPα (6h) and NPYR1 and 2 (24h) mRNAs were greater in NPY- than vehicle-injected chicks. At several times, adipose triglyceride lipase, MTTP, perilipin 1, NPYR1, and NPYR2 mRNAs were greater in LWS than HWS, while expression of SCD1, glycerol-3-phosphate acyltransferase 3 and lipoprotein lipase was greater in HWS than LWS. Thus, NPY promotes fat deposition and inhibits lipolysis in chicks, with line differences indicative of greater rates of lipolysis in LWS and adipogenesis in HWS.
神经肽Y（NPY）刺激食欲并促进脂质沉积。我们已经证明，对于体重较低（LWS）或较高（HWS）品系的雏鸡，其对中枢NPY的食物摄入反应存在差异敏感性，但尚未报道外周是否存在此类差异。在5日龄时，给LWS和HWS雏鸡腹腔注射0（溶剂）、60或120μg/kg体重的NPY，并在1、3、6、12和24小时收集皮下脂肪组织和血浆（n = 12）。注射NPY后，在1和3小时甘油-3-磷酸脱氢酶（G3PDH）活性增加，在1和12小时血浆非酯化脂肪酸（NEFAs）减少。HWS雏鸡的G3PDH活性高于LWS雏鸡，而LWS雏鸡的NEFAs含量更高。在1小时时，注射NPY的雏鸡中过氧化物酶体增殖物激活受体γ（PPARγ）、CCAAT/增强子结合蛋白（C/EBP）α和微粒体甘油三酯转移蛋白（MTTP）的mRNA水平降低，而NPY受体1（NPYR1）增加。在1小时时，NPY使HWS雏鸡的硬脂酰辅酶A去饱和酶（SCD1）表达增加，但LWS雏鸡未增加。与注射溶剂的雏鸡相比，注射NPY的雏鸡中PPARγ（3和6小时）、C/EBPβ（3小时）、C/EBPα（6小时）以及NPYR1和NPYR2（24小时）的mRNA水平更高。在多个时间点，LWS雏鸡的脂肪甘油三酯脂肪酶、MTTP、围脂滴蛋白1、NPYR1和NPYR2的mRNA水平高于HWS雏鸡，而SCD1、甘油-3-磷酸酰基转移酶3和脂蛋白脂肪酶的表达在HWS雏鸡中高于LWS雏鸡。因此，NPY促进雏鸡脂肪沉积并抑制脂肪分解，品系差异表明LWS雏鸡的脂肪分解率更高，而HWS雏鸡的脂肪生成率更高。
