用等压质量标签对突触蛋白的 S-亚硝基化蛋白质组进行分析。
Proteome profiling of s-nitrosylated synaptosomal proteins by isobaric mass tags.
机构信息
German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany.
Institute of Biochemistry and Molecular Biology, University of Bonn, Germany.
出版信息
J Neurosci Methods. 2017 Nov 1;291:95-100. doi: 10.1016/j.jneumeth.2017.08.005. Epub 2017 Aug 5.
Abstract
• Protocol for quantitative proteomics of nitrosylation on synaptosomal proteins. • Identification of endogenous nitrosylation independent of induction by NO donors. • Use of iodoTMT sixplex mass tags for stable labeling, enrichment, identification, and multiplex quantitation. • Applicable on low amounts of sample material of mouse and human brain tissue.
摘要
• 突触蛋白硝化作用的定量蛋白质组学研究方案。 • 无需通过 NO 供体诱导即可鉴定内源性硝化作用。 • 使用碘代 TMT 六重标记物进行稳定标记、富集、鉴定和多重定量。 • 适用于小鼠和人脑组织等少量样本材料。
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