Ni Wenjun, Song Erlin, Gong Mancheng, Li Yongxiang, Yao Jie, An Ruihua
Department of Urology Surgery, The First Affiliated Hospital of Harbin Medical University.
Department of Urology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang.
Onco Targets Ther. 2017 Jun 19;10:3039-3047. doi: 10.2147/OTT.S137641. eCollection 2017.
Renal cell carcinoma (RCC) is a common type of kidney cancer. Normally, surgical treatment can prolong life, but only for patients with early stage tumors. However, it is difficult for early detection strategies to distinguish between benign and malignant kidney tumors. Therefore, potential biomarkers for early diagnosis and prognosis of RCC are needed. Intriguingly, mounting evidence has demonstrated that many long noncoding RNAs (lncRNAs) are strongly linked to cancers. Indeed, promising RCC-associated lncRNA biomarkers have also been identified. However, the functional and prognostic roles of the antisense (AS) serum deprivation response () lncRNA (SDPR-AS) in RCC remain largely unknown. The aims of this study were to investigate the expression and prognostic relevance of SDPR-AS in RCC. We uncovered the downregulated expressions of both lncRNA SDPR-AS and its protein-coding gene, , in RCC tissues compared to the matched normal tissues. Furthermore, SDPR-AS and expressions were positively correlated. Overexpression and knockdown experiments suggested that SDPR-AS and were coregulated in RCC cell lines. In addition, overexpression of SDPR-AS suppressed cell migration and invasion, but not cell growth. Furthermore, expression of SDPR-AS was associated with tumor differentiation and lymphatic metastasis. Kaplan-Meier survival and log-rank tests demonstrated the association of elevated expression of SDPR-AS with increased overall survival. In conclusion, our results suggest that the SDPR-AS may serve as a prognostic biomarker and therapeutic target of RCC.
肾细胞癌（RCC）是一种常见的肾癌类型。通常，手术治疗可以延长生命，但仅适用于早期肿瘤患者。然而，早期检测策略很难区分良性和恶性肾肿瘤。因此，需要用于RCC早期诊断和预后的潜在生物标志物。有趣的是，越来越多的证据表明许多长链非编码RNA（lncRNA）与癌症密切相关。事实上，也已经鉴定出有前景的RCC相关lncRNA生物标志物。然而，反义（AS）血清剥夺反应（）lncRNA（SDPR-AS）在RCC中的功能和预后作用仍 largely未知。本研究的目的是调查SDPR-AS在RCC中的表达及其与预后的相关性。我们发现与匹配的正常组织相比，RCC组织中lncRNA SDPR-AS及其蛋白质编码基因的表达均下调。此外，SDPR-AS和的表达呈正相关。过表达和敲低实验表明，SDPR-AS和在RCC细胞系中是共调节的。此外，SDPR-AS的过表达抑制细胞迁移和侵袭，但不抑制细胞生长。此外，SDPR-AS的表达与肿瘤分化和淋巴转移有关。Kaplan-Meier生存分析和对数秩检验表明，SDPR-AS表达升高与总生存期增加有关。总之，我们的结果表明，SDPR-AS可能作为RCC的预后生物标志物和治疗靶点。
