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基于牙种植体螺纹表面对种植体周围炎细菌黏附的体外激光治疗平台构建

In Vitro Laser Treatment Platform Construction with Dental Implant Thread Surface on Bacterial Adhesion for Peri-Implantitis.

作者信息

Kuo Hsien-Nan, Mei Hsiang-I, Liu Tung-Kuan, Liu Tse-Ying, Lo Lun-Jou, Lin Chun-Li

机构信息

Institute of Engineering Science and Technology, National Kaohsiung First University of Science and Technology, Kaohsiung, Taiwan.

Department of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan.

出版信息

Biomed Res Int. 2017;2017:4732302. doi: 10.1155/2017/4732302. Epub 2017 Jul 16.

Abstract

This study constructs a standard in vitro laser treatment platform with dental implant thread surface on bacterial adhesion for peri-implantitis at different tooth positions. The standard clinical adult tooth jaw model was scanned to construct the digital model with 6 mm bone loss depth on behalf of serious peri-implantitis at the incisor, first premolar, and first molar. A cylindrical suite connected to the implant and each tooth root in the jaw model was designed as one experimental unit set to allow the suite to be replaced for individual bacterial adhesion. The digital peri-implantitis and suite models were exported to fulfill the physical model using ABS material in a 3D printer. A 3 mm diameter specimen implant on bacterial adhesion against was performed for gram-negative bacteria. An Er:YAG laser, working with a chisel type glass tip, was moved from the buccal across the implant thread to the lingual for about 30 seconds per sample to verify the in vitro laser treatment platform. The result showed that the sterilization rate can reach 99.3% and the jaw model was not damaged after laser irradiation testing. This study concluded that using integrated image processing, reverse engineering, CAD system, and a 3D printer to construct a peri-implantitis model replacing the implant on bacterial adhesion and acceptable sterilization rate proved the feasibility of the proposed laser treatment platform.

摘要

本研究构建了一个标准的体外激光治疗平台,该平台带有牙种植体螺纹表面,用于研究不同牙位的种植体周围炎的细菌黏附情况。对标准临床成人牙颌模型进行扫描,构建骨丧失深度为6毫米的数字模型,分别代表切牙、第一前磨牙和第一磨牙处严重的种植体周围炎。在颌骨模型中,将连接种植体和每个牙根的圆柱形套件设计为一个实验单元组,以便能够更换套件以进行个体细菌黏附实验。将数字种植体周围炎模型和套件模型导出,使用3D打印机用ABS材料制作物理模型。对直径3毫米的标本种植体进行革兰氏阴性菌黏附实验。使用带有凿型玻璃尖端的铒激光,从颊侧穿过种植体螺纹向舌侧移动,每个样本照射约30秒,以验证体外激光治疗平台。结果表明,杀菌率可达99.3%,且激光照射测试后颌骨模型未受损。本研究得出结论,利用图像处理、逆向工程、CAD系统和3D打印机构建种植体周围炎模型,用于替代种植体进行细菌黏附实验,并获得可接受的杀菌率,证明了所提出的激光治疗平台的可行性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/349d/5534282/7f9d50a8f343/BMRI2017-4732302.001.jpg

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