Department of Otolaryngology‑Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.
Department of Pathology, The First Hospital Affiliated to Bengbu Medical College, Bengbu, Anhui 233000, P.R. China.
Mol Med Rep. 2017 Oct;16(4):4934-4940. doi: 10.3892/mmr.2017.7187. Epub 2017 Aug 7.
Previous studies have demonstrated that elevated yes‑associated protein (YAP) expression is associated with tumor aggression and poor prognosis in various types of human cancer. However, the clinicopathological significance and the prognostic value of YAP in laryngeal squamous cell carcinoma (LSCC) is unknown. The aim of the present study was to identify the expression pattern and prognostic significance of YAP in patients with LSCC. YAP mRNA and protein expression levels were examined in fresh and archived LSCC samples using the reverse transcription‑quantitative polymerase chain reaction, immunohistochemistry (IHC) and western blotting. The association between YAP expression levels with the malignant status and prognosis of patients with LSCC was analyzed. Upregulated protein and mRNA expression levels of YAP were detected in LSCC tissues compared with paired healthy surgical margin tissues. Positive expression of YAP was identified in 84/121 (69.4%) LSCC tissues and in 4/30 (13.3%) healthy surgical margin tissues by IHC. Positive YAP protein expression was significantly associated with clinical stage, TNM classification, lymph node metastasis and differentiated degree. Patients with positive YAP expression exhibited a significantly decreased overall survival time compared with patients with negative YAP expression (P=0.0002). Multivariate analysis indicated that the level of YAP expression was an independent prognostic factor for poor survival in patients with LSCC (P=0.012). In conclusion, the expression level of YAP was significantly increased in LSCC and associated with the malignant status of LSCC. Therefore, YAP may represent a novel biomarker for predicting the prognosis of patients with LSCC.
先前的研究表明，在各种类型的人类癌症中，YAP（Yes 相关蛋白）的表达升高与肿瘤侵袭性和不良预后相关。然而，YAP 在喉鳞状细胞癌（LSCC）中的临床病理意义和预后价值尚不清楚。本研究旨在确定 YAP 在 LSCC 患者中的表达模式和预后意义。使用逆转录定量聚合酶链反应、免疫组织化学（IHC）和蛋白质印迹法检测新鲜和存档的 LSCC 样本中的 YAP mRNA 和蛋白表达水平。分析 YAP 表达水平与 LSCC 患者恶性状态和预后的关系。与配对的健康手术边缘组织相比，LSCC 组织中检测到 YAP 蛋白和 mRNA 的表达水平上调。IHC 检测到 84/121（69.4%）例 LSCC 组织和 4/30（13.3%）例健康手术边缘组织中存在 YAP 阳性表达。YAP 蛋白阳性表达与临床分期、TNM 分类、淋巴结转移和分化程度显著相关。与 YAP 阴性表达的患者相比，YAP 阳性表达的患者总生存时间明显缩短（P=0.0002）。多因素分析表明，YAP 表达水平是 LSCC 患者预后不良的独立预后因素（P=0.012）。总之，YAP 在 LSCC 中的表达水平显著升高，与 LSCC 的恶性状态相关。因此，YAP 可能代表预测 LSCC 患者预后的一种新的生物标志物。
