Kyallo Martina, Ateka Elijah Miinda, Sseruwagi Peter, Ascencio-Ibáñez José Trinidad, Ssemakula Mildred-Ochwo, Skilton Robert, Ndunguru Joseph
Biosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub, P.O. Box 30709-00100, Nairobi, Kenya.
Department of Horticulture, Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya.
Arch Virol. 2017 Nov;162(11):3439-3445. doi: 10.1007/s00705-017-3495-x. Epub 2017 Aug 9.
Weed-infecting begomoviruses play an important role in the epidemiology of crop diseases because they can potentially infect crops and contribute to the genetic diversity of crop-infecting begomoviruses. Despite the important epidemiological role that weed-infecting begomoviruses play, they remain insufficiently studied in Africa. Recently, we identified Deinbollia mosaic virus (DMV), a distinct begomovirus found naturally infecting the weed host Deinbollia borbonica (Sapindaceae) in Kenya and Tanzania. In this study, we investigated the capacity of DMV to infect a restricted host range of Solanaceae and Euphorbiaceae species. Biolistic inoculation of Nicotiana benthamiana with concatemeric DNAs resulted in systemic infection associated with yellow mosaic symptoms, while DNA partial dimers caused asymptomatic systemic infection. DMV was not infectious to cassava (Manihot esculenta Crantz), suggesting host resistance to the virus. Here, we demonstrate the first experimental infectivity analysis of DMV in N. benthamiana and cassava.
侵染杂草的双生病毒在作物病害流行病学中发挥着重要作用，因为它们有可能感染作物，并增加侵染作物的双生病毒的遗传多样性。尽管侵染杂草的双生病毒在流行病学中具有重要作用，但在非洲对它们的研究仍然不足。最近，我们鉴定出了代因博利亚花叶病毒（DMV），这是一种独特的双生病毒，在肯尼亚和坦桑尼亚自然感染杂草寄主博氏韶子（无患子科）。在本研究中，我们调查了DMV感染茄科和大戟科有限寄主范围物种的能力。用串联DNA对本氏烟草进行基因枪接种导致系统感染并伴有黄色花叶症状，而DNA部分二聚体则引起无症状系统感染。DMV对木薯（Manihot esculenta Crantz）无感染性，表明寄主对该病毒具有抗性。在此，我们展示了DMV在本氏烟草和木薯中的首次实验感染性分析。
