Kyallo Martina, Sseruwagi Peter, Skilton Robert A, Ochwo-Ssemakula Mildred, Wasswa Peter, Ndunguru Joseph
Biosciences Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, P.O. Box 30709-00100, Nairobi, Kenya.
Mikocheni Agricultural Research Institute, P.O. Box 6226, Dar es Salaam, Tanzania.
Arch Virol. 2017 May;162(5):1393-1396. doi: 10.1007/s00705-016-3217-9. Epub 2017 Jan 9.
Four isolates of a bipartite begomovirus from naturally infected Deinbollia borbonica plants exhibiting yellow mosaic symptoms in Kenya and Tanzania were molecularly characterised. The DNA-A was most closely related to that of tomato leaf curl Mayotte virus (AM701764; 82%), while the DNA-B shared the highest nucleotide sequence identity with that of East African cassava mosaic virus (AJ704953) at 65%. Based on the current ICTV species demarcation criterion for the genus Begomovirus (≥91% sequence identity for the complete DNA-A), we report the full-length genome sequence of this novel bipartite begomovirus. The results reveal additional diversity and reservoir hosts of begomoviruses in East Africa.
对从肯尼亚和坦桑尼亚自然感染的、表现出黄色花叶症状的波旁德氏木（Deinbollia borbonica）植株中分离出的四种双分体菜豆金色花叶病毒进行了分子特征分析。DNA-A与番茄卷叶马约特病毒（AM701764；82%）的关系最为密切，而DNA-B与东非木薯花叶病毒（AJ704953）的核苷酸序列同一性最高，为65%。根据国际病毒分类委员会（ICTV）目前对菜豆金色花叶病毒属的种划分标准（完整DNA-A的序列同一性≥91%），我们报告了这种新型双分体菜豆金色花叶病毒的全长基因组序列。结果揭示了东非菜豆金色花叶病毒的更多多样性和宿主库。
