Department of Neurology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.
Department of Neurology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China.
Neurosci Bull. 2017 Oct;33(5):493-500. doi: 10.1007/s12264-017-0163-9. Epub 2017 Aug 8.
In this study, we conducted a clinical analysis of lymphocyte subtypes in 268 patients with Parkinson's disease (PD) to assess their clinical impact as a potential marker of advanced PD in Chinese patients. The participants comprised 268 sporadic PD patients and 268 healthy controls. The numbers of natural killer (NK) cells and CD3+, CD3+CD4+, CD3+CD8+, and CD19+ lymphocytes from peripheral blood were determined by immunostaining and flow cytometric analysis and the percentages of these CD+ T cells were calculated. The ratio of regulatory T (Treg)/helper T 17 (Th17) lymphocytes from 64 PD patients and 46 controls was determined by flow cytometric analysis. The results showed that the percentage of NK cells was higher in advanced PD patients than in controls (22.92% ± 10.08% versus 19.76% ± 10.09%, P = 0.006), while CD3+ T cells are decreased (62.93% ± 9.27% versus 65.75% ± 9.13%, P = 0.005). The percentage of CD19+ B cells in male patients was lower (P = 0.021) than in female patients, whereas NK cells were increased (P < 0.0001). The scores on the Unified Parkinson's Disease Rating Scale (UPDRS) and the Non-Motor Symptoms Scale in late-onset PD patients were significantly higher than those in early-onset patients (P = 0.024 and P = 0.007, respectively). The percentage of CD19+ B cells in patients with UPDRS scores >24 was lower than in those with scores <24 (10.17% ± 4.19% versus 12.22% ± 5.39%, P = 0.009). In addition, the Treg/Th17 ratio in female patients was higher than that in female controls (13.88 ± 6.32 versus 9.94 ± 4.06, P = 0.042). These results suggest that the percentages of NK cells, CD3+ T cells, and CD19+ B cells along with the Treg/Th17 ratio in peripheral blood may be used to predict the risk of PD in Chinese individuals and provide fresh avenues for novel diagnostic biomarkers and therapeutic designs.
本研究对 268 例帕金森病（PD）患者的淋巴细胞亚群进行了临床分析，旨在评估其作为中国患者 PD 进展潜在标志物的临床意义。参与者包括 268 例散发性 PD 患者和 268 例健康对照者。通过免疫染色和流式细胞术分析测定外周血自然杀伤（NK）细胞和 CD3+、CD3+CD4+、CD3+CD8+和 CD19+淋巴细胞数量，并计算这些 CD+T 细胞的百分比。通过流式细胞术分析测定 64 例 PD 患者和 46 例对照者调节性 T（Treg）/辅助性 T17（Th17）淋巴细胞的比值。结果显示，晚期 PD 患者的 NK 细胞百分比高于对照组（22.92%±10.08%比 19.76%±10.09%，P=0.006），而 CD3+T 细胞减少（62.93%±9.27%比 65.75%±9.13%，P=0.005）。男性患者 CD19+B 细胞百分比较低（P=0.021），而 NK 细胞较高（P<0.0001）。晚发性 PD 患者的统一帕金森病评定量表（UPDRS）和非运动症状量表评分明显高于早发性患者（P=0.024 和 P=0.007）。UPDRS 评分>24 的患者的 CD19+B 细胞百分比低于评分<24 的患者（10.17%±4.19%比 12.22%±5.39%，P=0.009）。此外，女性患者的 Treg/Th17 比值高于女性对照者（13.88%±6.32 比 9.94%±4.06，P=0.042）。这些结果表明，外周血 NK 细胞、CD3+T 细胞和 CD19+B 细胞的百分比以及 Treg/Th17 比值可能用于预测中国个体 PD 的发病风险，并为新的诊断生物标志物和治疗设计提供新的途径。
