Kwon Chunga, Kim Youngjun, Jeon Hojeong
Korea Institute of Science and Technology Europe (KIST-Europe) Forschungsgesellschaft mbH, Campus E 7 1, 66123 Saarbrücken, Germany.
Department of Biomedical Engineering, University of Science and Technology (UST), Daejeon 34113, Korea.
J Funct Biomater. 2017 Aug 9;8(3):34. doi: 10.3390/jfb8030034.
Herein, a micro-patterned cell adhesive surface is prepared for the future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro patterns were prepared by a photolithography process. We investigated the effect of microscale topographical patterned surfaces on decreasing the collective cell migration rate. PDMS substrates were prepared through soft lithography using Si molds fabricated by photolithography. Afterwards, we observed the collective cell migration of human lens epithelial cells (B-3) on various groove/ridge patterns and evaluated the migration rate to determine the pattern most effective in slowing down the cell sheet spreading speed. Microgroove patterns were variable, with widths of 3, 5, and 10 µm. After the seeding, time-lapse images were taken under controlled cell culturing conditions. Cell sheet borders were drawn in order to assess collective migration rate. Our experiments revealed that the topographical patterned surfaces could be applied to intraocular lenses to prevent or slow the development of posterior capsular opacification (PCO) by delaying the growth and spread of human lens epithelial cells.
在此，为未来的医疗设备设计制备了一种微图案化的细胞粘附表面。通过光刻工艺制备了一维聚二甲基硅氧烷（PDMS）微图案。我们研究了微尺度拓扑图案化表面对降低细胞集体迁移速率的影响。PDMS基底通过软光刻使用光刻制造的硅模具制备。之后，我们观察了人晶状体上皮细胞（B - 3）在各种沟槽/脊图案上的集体迁移，并评估迁移速率以确定在减缓细胞片层铺展速度方面最有效的图案。微沟槽图案的宽度分别为3、5和10微米，各不相同。接种后，在受控的细胞培养条件下拍摄延时图像。绘制细胞片层边界以评估集体迁移速率。我们的实验表明，拓扑图案化表面可应用于人工晶状体，通过延迟人晶状体上皮细胞的生长和扩散来预防或减缓后囊膜混浊（PCO）的发展。
Zotero 插件
