Melnichuk Nataliia, Semernikova Larisa, Tkachuk Zenoviy
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 03680 Kyiv, Ukraine.
Pharmaceuticals (Basel). 2017 Aug 9;10(3):71. doi: 10.3390/ph10030071.
The influenza virus hemagglutinin (HA) mediates both receptor (glycan) binding and membrane fusion for cell entry and has been the basis for subtyping influenza viruses. The oligoribonucleotides-d-mannitol (ORNs-d-M) complexes possess an anti-influenza activity in vitro and in vivo. In the present studies, we have found that ORNs-d-M interferes with hemagglutinin (HA)-glycan interaction and suppress viral infection in host cells. HA-glycan interactions were evaluated to indirectly quantify the amount of influenza virus titer by an agglutination assay. Influenza virus infectivity was determined by TCID assay. The direct virucidal action of the complexes was evaluated by both cytopathic effects (CPE) reduction assay and cell MTT assay. We found that ORNs-d-M hinders interaction between HA and glycan. These complexes decreased the infectivity of influenza virus and had a direct virucidal action. ORNs-d-M reduces influenza virus infectivity, affecting the HA-glycan interaction in vitro. By suppressing the influenza viral infection, the ORNs-d-M can have direct virucidal action.
流感病毒血凝素（HA）介导受体（聚糖）结合和膜融合以实现细胞进入，并且一直是流感病毒亚型划分的基础。寡核糖核苷酸 - d - 甘露醇（ORNs - d - M）复合物在体外和体内均具有抗流感活性。在本研究中，我们发现ORNs - d - M会干扰血凝素（HA）与聚糖的相互作用，并抑制宿主细胞中的病毒感染。通过凝集试验评估HA - 聚糖相互作用，以间接定量流感病毒滴度。通过TCID试验确定流感病毒的感染性。通过细胞病变效应（CPE）降低试验和细胞MTT试验评估复合物的直接杀病毒作用。我们发现ORNs - d - M会阻碍HA与聚糖之间的相互作用。这些复合物降低了流感病毒的感染性，并具有直接的杀病毒作用。ORNs - d - M降低流感病毒感染性，在体外影响HA - 聚糖相互作用。通过抑制流感病毒感染，ORNs - d - M可具有直接杀病毒作用。
