Pilcher W H, Joseph S A
Peptides. 1986 Sep-Oct;7(5):783-9. doi: 10.1016/0196-9781(86)90096-3.
The distribution of opiocortin- (OP-ir) and tyrosine hydroxylase-immunoreactive (TH-ir) perikarya was examined immunocytochemically in rats treated neonatally with the neurotoxin monosodium glutamate (MSG). While OP-ir and TH-ir perikarya were eliminated in the arcuate nucleus in treated animals, the OP-ir and TH-ir cell groups of the nucleus tractus solitarius and contiguous medullary regions were unaffected. This selective elimination of arcuate neurons permitted us to examine specifically the fiber projections of the medullary OP-ir perikarya in treated animals. This revealed a preferential distribution of delicate fibers originating in NTS, to discrete medullary and pontine areas. In control animals, these same terminal fields appeared to be more densely populated with an additional population of thicker OP-ir fibers, suggesting the possibility of a shared innervation of these brainstem regions by both hypothalamic and medullary OP-ir neurons.
采用免疫细胞化学方法，对新生期经神经毒素谷氨酸单钠（MSG）处理的大鼠中阿片皮质素免疫反应性（OP-ir）和酪氨酸羟化酶免疫反应性（TH-ir）核周体的分布进行了研究。虽然在经处理的动物中，弓状核内的OP-ir和TH-ir核周体被消除，但孤束核及相邻延髓区域的OP-ir和TH-ir细胞群未受影响。弓状神经元的这种选择性消除使我们能够专门研究经处理动物中延髓OP-ir核周体的纤维投射。这揭示了起源于孤束核的纤细纤维向离散的延髓和脑桥区域的优先分布。在对照动物中，这些相同的终末区域似乎有更多较粗的OP-ir纤维，表明下丘脑和延髓OP-ir神经元可能共同支配这些脑干区域。
