Zakrzewska Karolina Ewa, Samluk Anna, Wencel Agnieszka, Dudek Krzysztof, Pijanowska Dorota Genowefa, Pluta Krzysztof Dariusz
Department of Hybrid Microbiosystems Engineering, Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw, Poland.
Chair and Department of General, Transplant and Liver Surgery, Medical University of Warsaw, Warsaw, Poland.
PLoS One. 2017 Aug 9;12(8):e0182846. doi: 10.1371/journal.pone.0182846. eCollection 2017.
Cell-based therapies that could provide an alternative treatment for the end-stage liver disease require an adequate source of functional hepatocytes. There is little scientific evidence for the influence of patient's age, sex, and chemotherapy on the cell isolation efficiency and metabolic activity of the harvested hepatocytes. The purpose of this study was to investigate whether hepatocytes derived from different sources display differential viability and biosynthetic capacity. Liver cells were isolated from 41 different human tissue specimens. Hepatocytes were labeled using specific antibodies and analyzed using flow cytometry. Multiparametric analysis of the acquired data revealed statistically significant differences between some studied groups of patients. Generally, populations of cells isolated from the male specimens had greater percentage of biosynthetically active hepatocytes than those from the female ones regardless of age and previous chemotherapy of the patient. Based on the albumin staining (and partially on the α-1-antitrypsin labeling) after donor liver exclusion (6 out of 41 samples), our results indicated that: 1. samples obtained from males gave a greater percentage of active hepatocytes than those from females (p = 0.034), and 2. specimens from the males after chemotherapy greater than those from the treated females (p = 0.032).
能够为终末期肝病提供替代治疗的细胞疗法需要充足的功能性肝细胞来源。关于患者年龄、性别和化疗对收获的肝细胞的细胞分离效率和代谢活性的影响，几乎没有科学证据。本研究的目的是调查来自不同来源的肝细胞是否表现出不同的活力和生物合成能力。从41个不同的人体组织标本中分离肝细胞。使用特异性抗体对肝细胞进行标记，并通过流式细胞术进行分析。对获取数据的多参数分析显示，一些研究的患者组之间存在统计学上的显著差异。总体而言，无论患者的年龄和既往化疗情况如何，从男性标本中分离的细胞群体中具有生物合成活性的肝细胞百分比高于从女性标本中分离的细胞群体。根据供体肝脏排除后（41个样本中的6个）的白蛋白染色（部分基于α-1-抗胰蛋白酶标记），我们的结果表明：1. 从男性获得的样本中活性肝细胞的百分比高于从女性获得的样本（p = 0.034），2. 化疗后男性的标本大于接受治疗的女性的标本（p = 0.032）。
