使用含全血和富血小板血浆的微固定钴铬膜对兔胫骨进行骨增量
Bone Augmentation in Rabbit Tibia Using Microfixed Cobalt-Chromium Membranes with Whole Blood and Platelet-Rich Plasma.
作者信息
Decco Oscar A, Beltrán Víctor, Zuchuat Jésica I, Cura Andrea C, Lezcano María F, Engelke Wilfried
机构信息
Department of Bioingenieering, Faculty of Engineering, National University of Entre Ríos, Route 11 Km.10, Oro Verde (Paraná)-Entre Ríos 3100, Argentina.
CIMOFIR Research Centre, Faculty of Dentistry, University of La Frontera, Temuco 4781176, Chile.
出版信息
Materials (Basel). 2015 Jul 30;8(8):4843-4856. doi: 10.3390/ma8084843.
BACKGROUND
Bone augmentation is a subject of intensive investigation in regenerative bone medicine and constitutes a clinical situation in which autogenous bone grafts or synthetic materials are used to aid new bone formation.
METHOD
Based on a non-critical defect, Co-Cr barrier membranes were placed on six adult Fauve de Bourgogne rabbits, divided into two groups: whole blood and PRP. Three densitometric controls were performed during the experiment. The animals were euthanized at 30, 45, 60, and 110 days. The presence of newly formed bone was observed. Samples for histological studies were taken from the augmentation center.
RESULTS
External and internal bone tissue augmentation was observed in almost all cases. Significant differences between PRP- and whole blood-stimulated bone augmentation were not observed. At 60 days, bones with PRP presented higher angiogenesis, which may indicate more proliferation and cellular activity.
CONCLUSION
PRP activates the bone regeneration process under optimized conditions by stimulation of osteoblast proliferation after six weeks, when a significant difference in cellular activity was observed. Membranes could stimulate bone augmentation at the site of placement and in the surrounding areas.
背景
骨增量是再生骨医学中深入研究的课题，是一种使用自体骨移植或合成材料辅助新骨形成的临床情况。
方法
基于非临界性骨缺损，将钴铬屏障膜置于6只成年布尔戈尼浅黄褐色兔上，分为两组：全血组和富血小板血浆（PRP）组。实验期间进行了三次骨密度对照。在30、45、60和110天时对动物实施安乐死。观察新形成骨的存在情况。从骨增量中心采集组织学研究样本。
结果
几乎在所有病例中均观察到外部和内部骨组织的增量。未观察到PRP刺激的骨增量与全血刺激的骨增量之间存在显著差异。在60天时，PRP处理的骨具有更高的血管生成，这可能表明有更多的增殖和细胞活性。
结论
在优化条件下，PRP通过在六周后刺激成骨细胞增殖来激活骨再生过程，此时观察到细胞活性存在显著差异。屏障膜可刺激植入部位及周围区域的骨增量。
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