Molecular identification of species using the A gene: Identificación molecular de especies de utilizando el gen .

Currently for bacterial identification and classification the gene encoding 16S rRNA is used as a reference method for the analysis of strains of the genus However, it does not have enough polymorphism to differentiate them at the species level. This fact makes it necessary to search for molecular targets that can provide better identification. The A gene (encoding the enzyme superoxide dismutase) has had good results in identifying species of other In this study the A gene is proposed for the identification and differentiation at the species level of the genus We used 41 type species of various collections; a 386 bp fragment of the A gene was amplified and sequenced, and a phylogenetic analysis was performed comparing the genes (1171 bp), 65 (401 bp), A1 (494 bp), B (1195 bp) and B (401 bp). The sequences were aligned using the Clustal X program. Evolutionary trees according to the neighbour-joining method were created with the programs Phylo_win and MEGA 6. The specific variability of the A genus of the genus was analysed. A high phylogenetic resolution, significant genetic variability, and specificity and reliability were observed for the differentiation of the isolates at the species level. The polymorphism observed in the A gene sequence contains variable regions that allow the discrimination of closely related species. The clear specificity, despite its small size, proves to be of great advantage for use in taxonomic studies and clinical diagnosis of the genus