Glazunova Olga O, Raoult Didier, Roux Véronique
Laboratoire de Bactériologie-Virologie, Hôpital de la Timone, CNRS UMR 6236, IFR48, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2317-22. doi: 10.1099/ijs.0.005488-0. Epub 2009 Jul 20.
Phylogenetic analysis and species identification of members of the genus Streptococcus were carried out using partial sequence comparison of the 16S rRNA gene (1468-1478 bp), rpoB, encoding the beta subunit of RNA polymerase (659-680 bp), sodA, encoding the manganese-dependent superoxide dismutase (435-462 bp), groEL, encoding the 60 kDa heat-shock protein (757 bp), and gyrB, encoding the Beta subunit of DNA gyrase (458-461 bp). For the first time, most species within the genus Streptococcus were represented in the study (65 strains, representing 58 species and nine subspecies). Phylogenies inferred from rpoB, sodA, gyrB and groEL sequence comparisons were more discriminative than those inferred from 16S rRNA gene sequence comparison, and showed common clusters. The minimal interspecies divergence was 0.3, 2.7, 0, 2.5 and 3.4 % for the 16S rRNA gene, rpoB, sodA, gyrB and groEL, respectively. In general, groEL partial gene sequence comparison represented the best tool for identifying species and subspecies and for phylogenetic analysis.
利用16S rRNA基因（1468 - 1478 bp）、编码RNA聚合酶β亚基的rpoB（659 - 680 bp）、编码锰依赖性超氧化物歧化酶的sodA（435 - 462 bp）、编码60 kDa热休克蛋白的groEL（757 bp）以及编码DNA促旋酶β亚基的gyrB（458 - 461 bp）的部分序列比较，对链球菌属成员进行了系统发育分析和物种鉴定。该研究首次涵盖了链球菌属内的大多数物种（65株，代表58个物种和9个亚种）。从rpoB、sodA、gyrB和groEL序列比较推断出的系统发育关系比从16S rRNA基因序列比较推断出的更具区分性，并显示出共同的聚类。16S rRNA基因、rpoB、sodA、gyrB和groEL的种间最小差异分别为0.3%、2.7%、0%、2.5%和3.4%。总体而言，groEL部分基因序列比较是鉴定物种和亚种以及进行系统发育分析的最佳工具。
