Fan Lili, Li Zhijun, Huang Jiali, Yang Zengqi, Xiao Sa, Wang Xinglong, Dang Ruyi, Zhang Shuxia
College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Arch Virol. 2017 Nov;162(11):3447-3458. doi: 10.1007/s00705-017-3504-0. Epub 2017 Aug 9.
Avian encephalomyelitis (AE) is an important infectious poultry disease worldwide that is caused by avian encephalomyelitis virus (AEV). However, to date, the dynamic distribution of AEV in quails has not been well described. Quantitative real-time polymerase chain reaction (qPCR) and immunohistochemistry (IHC) assays were used to investigate the dynamic distribution and tissue tropism of AEV in experimentally infected Korean quail. AEV was detected in the cerebrum, cerebellum, proventriculus, intestine, liver, pancreas, spleen, bursa, lung and kidney as early as 3 days post-infection (dpi). The viral loads in the proventriculus, intestine, spleen and bursa were relatively higher than in other tissues. According to the qPCR results, AEV XY/Q-1410 infection lasted for at least 60 days in infected Korean quail. Immunohistochemistry-positive staining signals of AEV antigen were analysed by Image-Pro Plus software. A positive correlation between qPCR and IHC results was identified in most tissues. Our results provide an insight into the dynamic distribution of AEV in various tissues after infection. The distinct dynamic distribution of the viral genome in Korean quail in the early and late stages of infection suggests that AEV replication is affected by antibody levels and the maturity of the immune system of the host.
禽脑脊髓炎（AE）是一种在全球范围内重要的家禽传染性疾病，由禽脑脊髓炎病毒（AEV）引起。然而，迄今为止，AEV在鹌鹑体内的动态分布尚未得到充分描述。本研究采用定量实时聚合酶链反应（qPCR）和免疫组织化学（IHC）检测方法，对实验感染的韩国鹌鹑中AEV的动态分布和组织嗜性进行了研究。早在感染后3天（dpi），就在大脑、小脑、腺胃、肠道、肝脏、胰腺、脾脏、法氏囊、肺和肾脏中检测到了AEV。腺胃、肠道、脾脏和法氏囊中的病毒载量相对高于其他组织。根据qPCR结果，AEV XY/Q-1410在感染的韩国鹌鹑中持续感染至少60天。通过Image-Pro Plus软件分析AEV抗原的免疫组织化学阳性染色信号。在大多数组织中，qPCR和IHC结果之间存在正相关。我们的研究结果为感染后AEV在各种组织中的动态分布提供了见解。感染早期和晚期韩国鹌鹑中病毒基因组的不同动态分布表明，AEV的复制受宿主抗体水平和免疫系统成熟度的影响。
