Goto Yusuke, Yaegashi Gakuji, Kumagai Yoshihiro, Ogasawara Fusae, Goto Makiko, Mase Masaji
Iwate Prefecture Central Livestock Hygiene Service Center, 390-5 Sunakomi, Takizawa, Iwate 020-0605, Japan.
National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
J Vet Med Sci. 2019 Jan 25;81(1):103-106. doi: 10.1292/jvms.18-0550. Epub 2018 Nov 21.
A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for broadly detecting the avian encephalomyelitis virus (AEV). The new primers were based on conserved sequences of the 5'-untranslated region of AEV, because the virus was not detected using previous reported RT-PCR. By applying this method to the chicken samples with suspected AEV infection in Japan, we successfully obtained PCR products of the predicted size from all samples, and we confirmed the presence of AEV via sequence analysis.
开发了一种逆转录-聚合酶链反应（RT-PCR）方法，用于广泛检测禽脑脊髓炎病毒（AEV）。新引物基于AEV 5'-非翻译区的保守序列，因为使用先前报道的RT-PCR未检测到该病毒。通过将该方法应用于日本疑似感染AEV的鸡样本，我们成功地从所有样本中获得了预测大小的PCR产物，并通过序列分析证实了AEV的存在。
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