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在新生鼠下丘脑中表达 Prominin-1 的细胞分化为神经元和神经胶质细胞。

Cells Expressing Prominin-1 in Neonatal Murine Inferior Colliculus Differentiate into Neurons and Glia.

机构信息

Department of Otolaryngology, Kansai Medical University|, Osaka, Japan.

Regeneration Research Center for Intractable Diseases, Kansai Medical University, Osaka, Japan.

出版信息

Mol Neurobiol. 2018 Jun;55(6):4998-5005. doi: 10.1007/s12035-017-0701-5. Epub 2017 Aug 9.

DOI:10.1007/s12035-017-0701-5
PMID:28795331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5948249/
Abstract

Inferior colliculus (IC) is a major center for the integration and processing of acoustic information from ascending auditory pathways. Damage to the IC as well as normal aging can impair auditory function. Novel strategies such as stem cell (SC)-based regenerative therapy are required for functional recovery because mature neural cells have a minimal regenerative capacity after an injury. However, it is not known if there are neural stem cells (NSCs) in the IC. Herein, we screened for NSCs by surface marker analysis using flow cytometry. Isolated IC cells expressing prominin-1 (CD133) exhibited the cardinal NSC properties self-renewal capacity, expression of known NSC markers (SOX2 and nestin), and multipotency. Prominin-1-expressing cells from neonatal IC generated neurospheres, and culture of these neurospheres in differentiation-conditioned medium gave rise to gamma-aminobutyric acid-ergic (GABAergic) neurons, astrocytes, and oligodendrocytes. The presence of NSC-like cells in the IC has important implications for understanding IC development and for potential regenerative therapy.

摘要

下丘脑中脑（IC）是整合和处理来自上行听觉通路的声学信息的主要中心。IC 的损伤以及正常衰老都会损害听觉功能。由于成熟的神经细胞在受伤后几乎没有再生能力，因此需要基于干细胞（SC）的再生治疗等新策略来实现功能恢复。但是，IC 中是否存在神经干细胞（NSC）尚不清楚。在这里，我们使用流式细胞术通过表面标记分析筛选 NSC。表达 Prominin-1（CD133）的分离 IC 细胞表现出自我更新能力、已知 NSC 标志物（SOX2 和 nestin）的表达以及多能性等 NSC 的主要特性。来自新生 IC 的 Prominin-1 表达细胞产生神经球，并且将这些神经球在分化条件培养基中培养会产生γ-氨基丁酸能（GABAergic）神经元、星形胶质细胞和少突胶质细胞。IC 中存在类似于 NSC 的细胞对于理解 IC 的发育和潜在的再生治疗具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f60/5948249/7989c83493e3/12035_2017_701_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f60/5948249/407994e500a6/12035_2017_701_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f60/5948249/9d731565ddd6/12035_2017_701_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f60/5948249/b541cdbd9dac/12035_2017_701_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f60/5948249/fd904034b624/12035_2017_701_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f60/5948249/7989c83493e3/12035_2017_701_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f60/5948249/407994e500a6/12035_2017_701_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f60/5948249/9d731565ddd6/12035_2017_701_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f60/5948249/b541cdbd9dac/12035_2017_701_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f60/5948249/fd904034b624/12035_2017_701_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f60/5948249/7989c83493e3/12035_2017_701_Fig5_HTML.jpg

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