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评估大鼠下丘脑中的神经发生潜力：从出生后早期到成年期。

Evaluation of the Neurogenic Potential in the Rat Inferior Colliculus from Early Postnatal Days Until Adulthood.

机构信息

Department of Oto-Rhino-Laryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, Comprehensive Hearing Center, Universitaetsklinikum Wuerzburg, Josef-Schneider-Strasse 11, D-97080, Wuerzburg, Germany.

出版信息

Mol Neurobiol. 2021 Feb;58(2):719-734. doi: 10.1007/s12035-020-02151-6. Epub 2020 Oct 3.

DOI:10.1007/s12035-020-02151-6
PMID:33011856
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7843480/
Abstract

Neural stem cells (NSCs) have been recently identified in the inferior colliculus (IC). These cells are of particular interest, as no casual therapeutic options for impaired neural structures exist. This research project aims to evaluate the neurogenic potential in the rat IC from early postnatal days until adulthood. The IC of rats from postnatal day 6 up to 48 was examined by neurosphere assays and histological sections. In free-floating IC cell cultures, neurospheres formed from animals from early postnatal to adulthood. The amount of generated neurospheres decreased in older ages and increased with the number of cell line passages. Cells in the neurospheres and the histological sections stained positively with NSC markers (Doublecortin, Sox-2, Musashi-1, Nestin, and Atoh1). Dissociated single cells from the neurospheres differentiated and were stained positively for the neural lineage markers β-III-tubulin, glial fibrillary acidic protein, and myelin basic protein. In addition, NSC markers (Doublecortin, Sox-2, CDK5R1, and Ascl-1) were investigated by qRT-PCR. In conclusion, a neurogenic potential in the rat IC was detected and evaluated from early postnatal days until adulthood. The identification of NSCs in the rat IC and their age-specific characteristics contribute to a better understanding of the development and the plasticity of the auditory pathway and might be activated for therapeutic use.

摘要

神经干细胞（NSCs）最近在下丘脑中被鉴定出来。这些细胞特别有趣，因为对于受损的神经结构，目前还没有可行的治疗方法。本研究旨在评估大鼠下丘脑中从出生后早期到成年期的神经发生潜力。通过神经球测定和组织学切片检查了出生后第 6 天到 48 天的大鼠下丘脑。来自早期到成年的动物在下丘脑的游离细胞培养物中形成了神经球。随着细胞系传代次数的增加，生成的神经球数量减少，而年龄较大的动物生成的神经球数量增加。神经球和组织学切片中的细胞用 NSC 标志物（Doublecortin、Sox-2、Musashi-1、Nestin 和 Atoh1）染色呈阳性。来自神经球的分离单细胞分化并对神经谱系标志物β-III-微管蛋白、神经胶质纤维酸性蛋白和髓鞘碱性蛋白呈阳性染色。此外，还通过 qRT-PCR 研究了 NSC 标志物（Doublecortin、Sox-2、CDK5R1 和 Ascl-1）。总之，从出生后早期到成年，在大鼠下丘脑中检测到并评估了神经发生潜力。在下丘脑中鉴定出的 NSCs 及其年龄特异性特征有助于更好地了解听觉通路的发育和可塑性，并可能被激活用于治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bd8/7843480/720d14c86eac/12035_2020_2151_Fig8_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bd8/7843480/94feaacef4be/12035_2020_2151_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bd8/7843480/720d14c86eac/12035_2020_2151_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bd8/7843480/e6a2baacc36d/12035_2020_2151_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bd8/7843480/ea8e0c39fede/12035_2020_2151_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bd8/7843480/e8deec7db8b1/12035_2020_2151_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bd8/7843480/7328673abbb1/12035_2020_2151_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bd8/7843480/69c30329da58/12035_2020_2151_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bd8/7843480/939e0f4cc62c/12035_2020_2151_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bd8/7843480/94feaacef4be/12035_2020_2151_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bd8/7843480/720d14c86eac/12035_2020_2151_Fig8_HTML.jpg

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