Wemmer D E, Kumar N V, Metrione R M, Lazdunski M, Drobny G, Kallenbach N R
Biochemistry. 1986 Nov 4;25(22):6842-9. doi: 10.1021/bi00370a017.
Toxin II from Radianthus paumotensis (RpII) has been investigated by high-resolution NMR and chemical sequencing methods. Resonance assignments have been obtained for this protein by the sequential approach. NMR assignments could not be made consistent with the previously reported primary sequence for this protein, and chemical methods have been used to determine a sequence with which the NMR data are consistent. Analysis of the 2D NOE spectra shows that the protein secondary structure is comprised of two sequences of beta-sheet, probably joined into a distorted continuous sheet, connected by turns and extended loops, without any regular alpha-helical segments. The residues previously implicated in activity in this class of proteins, D8 and R13, occur in a loop region.
已通过高分辨率核磁共振（NMR）和化学测序方法对来自波莫图群岛放射海葵（Radianthus paumotensis）的毒素II（RpII）进行了研究。通过序列分析方法已获得该蛋白质的共振归属。NMR归属与先前报道的该蛋白质一级序列不一致，因此已使用化学方法确定与NMR数据一致的序列。对二维NOE谱的分析表明，该蛋白质二级结构由两个β-折叠序列组成，可能连接成一个扭曲的连续片层，通过转角和延伸环相连，没有任何规则的α-螺旋片段。先前认为与这类蛋白质活性有关的残基D8和R13位于一个环区。
