Mahnir V M, Kozlovskaya E P, Kalinovsky A I
Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Vladivostok.
Toxicon. 1992 Nov;30(11):1449-56. doi: 10.1016/0041-0101(92)90520-f.
A very potent non-protein toxin was isolated from the sea anemone Radianthus macrodactylus with the use of chromatography on polytetrafluoroethylene, CM-Sephadex C-25 and by cation and anion exchange HPLC. The toxin was identified as palytoxin by u.v.-, i.r.- and 500 MHz 1H NMR spectroscopy. Its LD50 was 0.74 +/- 0.29 micrograms/kg by i.v. injection into mice. So far, palytoxin has been associated with zoanthids only. The toxin caused the loss of haemoglobin from erythrocytes but only in about 2 hr after the beginning of incubation, which is characteristic for palytoxin from zoanthids. Sea anemone palytoxin was divided into major and minor components by HPLC. The latter proved to be a product of degradation of palytoxin.
通过在聚四氟乙烯、CM - Sephadex C - 25上进行色谱分析以及阳离子和阴离子交换高效液相色谱法，从大海葵巨指海葵中分离出一种剧毒非蛋白质毒素。通过紫外光谱、红外光谱和500兆赫的1H核磁共振光谱鉴定该毒素为岩沙海葵毒素。通过静脉注射到小鼠体内，其半数致死量为0.74±0.29微克/千克。到目前为止，岩沙海葵毒素仅与群体海葵有关。该毒素导致红细胞中的血红蛋白流失，但仅在孵育开始约2小时后出现，这是群体海葵中岩沙海葵毒素的特征。通过高效液相色谱法将大海葵岩沙海葵毒素分为主要成分和次要成分。后者被证明是岩沙海葵毒素的降解产物。
