Sladek J R, Collier T J, Haber S N, Roth R H, Redmond D E
Brain Res Bull. 1986 Dec;17(6):809-18. doi: 10.1016/0361-9230(86)90092-4.
Dopamine and norepinephrine neuroblasts of the ventral mesencephalon, hypothalamus, and dorsolateral pons were transplanted from fetal African green monkeys into multiple brain sites in adult (host) African green monkeys. Tissue was grafted from both early and late gestational age fetuses. Immunohistochemical analysis, with antibodies to tyrosine hydroxylase, a marker of catecholamine-containing neurons, showed large numbers of transplanted catecholamine neurons in host cerebral cortex, corpus striatum and lateral ventricles up to 69 days after transplantation. Serial reconstructions revealed extensive outgrowth of neuronal processes from large numbers of transplanted neurons as well as expansion of the size of transplanted (solid) grafts of fetal brain tissue in the host brain. Some grafts extended from the caudate nucleus into the adjacent lateral ventricles or from the cerebral cortex into the underlying corpus callosum and ventricle. There were dense networks of varicose fibers emanating from the tyrosine hydroxylase positive neurons within intraparenchymal and intraventricular grafts. The size and shape of transplanted neurons retained characteristics common to catecholaminergic neurons from the dissected regions of fetal brain. Thus, a variety of fetal, catecholamine-containing neurons survive transplantation to primate brain and produce extensive neuritic outgrowths. Moreover, rejection of transplanted tissue was not apparent. These findings provide essential information on nerve cell grafting in a species closely related to humans as a prerequisite in the consideration of neural transplants as therapeutic measures in neurological disease.
将来自非洲绿猴胎儿的中脑腹侧、下丘脑和脑桥背外侧的多巴胺能和去甲肾上腺素能神经母细胞移植到成年（宿主）非洲绿猴的多个脑区。移植的组织取自不同孕期的胎儿。使用针对酪氨酸羟化酶（一种含儿茶酚胺神经元的标志物）的抗体进行免疫组织化学分析，结果显示，在移植后长达69天的时间里，宿主大脑皮层、纹状体和侧脑室中存在大量移植的儿茶酚胺能神经元。连续重建显示，大量移植神经元的神经突起广泛生长，同时宿主大脑中胎儿脑组织移植（实体）移植物的大小也有所增加。一些移植物从尾状核延伸至相邻的侧脑室，或者从大脑皮层延伸至下方的胼胝体和脑室。在脑实质内和脑室内的移植物中，酪氨酸羟化酶阳性神经元发出了密集的曲张纤维网络。移植神经元的大小和形状保留了来自胎儿脑解剖区域的儿茶酚胺能神经元的共同特征。因此，多种含儿茶酚胺的胎儿神经元在移植到灵长类动物大脑后能够存活，并产生广泛的神经突起生长。此外，未观察到移植组织的排斥现象。这些发现为与人类密切相关的物种中的神经细胞移植提供了重要信息，这是将神经移植作为神经疾病治疗措施加以考虑的前提条件。
