DuPont Pioneer, Hayward, CA, USA.
DuPont Pioneer, Johnston, IA, USA.
Plant Biotechnol J. 2018 Feb;16(2):649-659. doi: 10.1111/pbi.12806. Epub 2017 Oct 1.
The coleopteran insect western corn rootworm (WCR, Diabrotica virgifera virgifera) is an economically important pest in North America and Europe. Transgenic corn plants producing Bacillus thuringiensis (Bt) insecticidal proteins have been useful against this devastating pest, but evolution of resistance has reduced their efficacy. Here, we report the discovery of a novel insecticidal protein, PIP-47Aa, from an isolate of Pseudomonas mosselii. PIP-47Aa sequence shows no shared motifs, domains or signatures with other known proteins. Recombinant PIP-47Aa kills WCR, two other corn rootworm pests (Diabrotica barberi and Diabrotica undecimpunctata howardi) and two other beetle species (Diabrotica speciosa and Phyllotreta cruciferae), but it was not toxic to the spotted lady beetle (Coleomegilla maculata) or seven species of Lepidoptera and Hemiptera. Transgenic corn plants expressing PIP-47Aa show significant protection from root damage by WCR. PIP-47Aa kills a WCR strain resistant to mCry3A and does not share rootworm midgut binding sites with mCry3A or AfIP-1A/1B from Alcaligenes that acts like Cry34Ab1/Cry35Ab1. Our results indicate that PIP-47Aa is a novel insecticidal protein for controlling the corn rootworm pests.
鞘翅目昆虫西方玉米根虫（WCR，Diabrotica virgifera virgifera）是北美和欧洲具有重要经济意义的害虫。生产苏云金芽孢杆菌（Bt）杀虫蛋白的转基因玉米植株对这种破坏性害虫非常有效，但抗药性的进化降低了它们的功效。在这里，我们报告了从假单胞菌属（Pseudomonas）分离株中发现的一种新型杀虫蛋白 PIP-47Aa。PIP-47Aa 序列与其他已知蛋白没有共享的基序、结构域或特征。重组 PIP-47Aa 可杀死 WCR、另外两种玉米根虫害虫（Diabrotica barberi 和 Diabrotica undecimpunctata howardi）和另外两种甲虫（Diabrotica speciosa 和 Phyllotreta cruciferae），但对斑衣蜡蝉（Coleomegilla maculata）或七种鳞翅目和半翅目昆虫没有毒性。表达 PIP-47Aa 的转基因玉米植株对 WCR 造成的根损伤表现出显著的保护作用。PIP-47Aa 可杀死对 mCry3A 具有抗性的 WCR 菌株，并且与 mCry3A 或 Alcaligenes 中的 AfIP-1A/1B （与 Cry34Ab1/Cry35Ab1 作用相似）没有共同的根虫中肠结合位点。我们的结果表明，PIP-47Aa 是一种用于控制玉米根虫害虫的新型杀虫蛋白。
