• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

来自JAK2+骨髓增殖性肿瘤的间充质基质细胞（MSC）与正常MSC不同，并有助于维持肿瘤性造血。

Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis.

作者信息

Ramos Teresa L, Sánchez-Abarca Luis Ignacio, Rosón-Burgo Beatriz, Redondo Alba, Rico Ana, Preciado Silvia, Ortega Rebeca, Rodríguez Concepción, Muntión Sandra, Hernández-Hernández Ángel, De Las Rivas Javier, González Marcos, González Porras José Ramón, Del Cañizo Consuelo, Sánchez-Guijo Fermín

机构信息

Universidad de Salamanca-IBSAL-Hospital Universitario, Servicio de Hematología, Salamanca, Spain.

Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León, Salamanca, Spain.

出版信息

PLoS One. 2017 Aug 10;12(8):e0182470. doi: 10.1371/journal.pone.0182470. eCollection 2017.

DOI:10.1371/journal.pone.0182470
PMID:28796790
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5552029/
Abstract

There is evidence of continuous bidirectional cross-talk between malignant cells and bone marrow-derived mesenchymal stromal cells (BM-MSC), which favors the emergence and progression of myeloproliferative neoplastic (MPN) diseases. In the current work we have compared the function and gene expression profile of BM-MSC from healthy donors (HD-MSC) and patients with MPN (JAK2V617F), showing no differences in the morphology, proliferation and differentiation capacity between both groups. However, BM-MSC from MPN expressed higher mean fluorescence intensity (MIF) of CD73, CD44 and CD90, whereas CD105 was lower when compared to controls. Gene expression profile of BM-MSC showed a total of 169 genes that were differentially expressed in BM-MSC from MPN patients compared to HD-MSC. In addition, we studied the ability of BM-MSC to support the growth and survival of hematopoietic stem/progenitor cells (HSPC), showing a significant increase in the number of CFU-GM colonies when MPN-HSPC were co-cultured with MPN-MSC. Furthermore, MPN-MSC showed alteration in the expression of genes associated to the maintenance of hematopoiesis, with an overexpression of SPP1 and NF-kB, and a downregulation of ANGPT1 and THPO. Our results suggest that BM-MSC from JAK2+ patients differ from their normal counterparts and favor the maintenance of malignant clonal hematopoietic cells.

摘要

有证据表明恶性细胞与骨髓来源的间充质基质细胞（BM-MSC）之间存在持续的双向串扰，这有利于骨髓增殖性肿瘤（MPN）疾病的发生和发展。在当前的研究中，我们比较了健康供体的BM-MSC（HD-MSC）和MPN患者（JAK2V617F）的功能和基因表达谱，结果显示两组在形态、增殖和分化能力方面没有差异。然而，与对照组相比，MPN患者的BM-MSC表达的CD73、CD44和CD90的平均荧光强度（MIF）更高，而CD105则更低。BM-MSC的基因表达谱显示，与HD-MSC相比，MPN患者的BM-MSC共有169个基因表达存在差异。此外，我们研究了BM-MSC支持造血干/祖细胞（HSPC）生长和存活的能力，结果显示当MPN-HSPC与MPN-MSC共培养时，CFU-GM集落数量显著增加。此外，MPN-MSC在与造血维持相关的基因表达方面出现改变，SPP1和NF-κB过表达，而ANGPT1和THPO下调。我们的结果表明，JAK2+患者的BM-MSC与其正常对应物不同，有利于恶性克隆造血细胞的维持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/3361184795ff/pone.0182470.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/01dfc0b9ed4a/pone.0182470.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/89214465178b/pone.0182470.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/29eea7b2c8d6/pone.0182470.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/94204ea44368/pone.0182470.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/9e6e9ae5d25d/pone.0182470.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/80055b658f3a/pone.0182470.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/3361184795ff/pone.0182470.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/01dfc0b9ed4a/pone.0182470.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/89214465178b/pone.0182470.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/29eea7b2c8d6/pone.0182470.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/94204ea44368/pone.0182470.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/9e6e9ae5d25d/pone.0182470.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/80055b658f3a/pone.0182470.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8164/5552029/3361184795ff/pone.0182470.g007.jpg

相似文献

1
Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis.来自JAK2+骨髓增殖性肿瘤的间充质基质细胞（MSC）与正常MSC不同，并有助于维持肿瘤性造血。
PLoS One. 2017 Aug 10;12(8):e0182470. doi: 10.1371/journal.pone.0182470. eCollection 2017.
2
HDAC8 overexpression in mesenchymal stromal cells from JAK2+ myeloproliferative neoplasms: a new therapeutic target?JAK2+骨髓增殖性肿瘤间充质基质细胞中HDAC8的过表达：一个新的治疗靶点？
Oncotarget. 2017 Apr 25;8(17):28187-28202. doi: 10.18632/oncotarget.15969.
3
Effects of JAK1/2 inhibition on bone marrow stromal cells of myeloproliferative neoplasm (MPN) patients and healthy individuals.JAK1/2 抑制对骨髓基质细胞的影响，包括骨髓增生性肿瘤（MPN）患者和健康个体。
Eur J Haematol. 2018 Jul;101(1):57-67. doi: 10.1111/ejh.13079. Epub 2018 Apr 30.
4
Alteration Analysis of Bone Marrow Mesenchymal Stromal Cells from De Novo Acute Myeloid Leukemia Patients at Diagnosis.初诊时新发急性髓系白血病患者骨髓间充质基质细胞的改变分析
Stem Cells Dev. 2017 May 15;26(10):709-722. doi: 10.1089/scd.2016.0295. Epub 2017 Apr 10.
5
Mesenchymal stromal cells from primary osteosarcoma are non-malignant and strikingly similar to their bone marrow counterparts.原发性骨肉瘤中的间充质基质细胞是非恶性的，与骨髓中的间充质基质细胞非常相似。
Int J Cancer. 2011 Jul 15;129(2):319-30. doi: 10.1002/ijc.25697. Epub 2010 Dec 1.
6
Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials.具有造血支持功能及其他潜能的人脐带间充质干细胞的分离与鉴定
Haematologica. 2006 Aug;91(8):1017-26. Epub 2006 Jul 25.
7
Hypoxia inhibits JAK2V617F activation via suppression of SHP-2 function in myeloproliferative neoplasm cells.低氧通过抑制骨髓增殖性肿瘤细胞中 SHP-2 的功能来抑制 JAK2V617F 的激活。
Exp Hematol. 2014 Sep;42(9):783-92.e1. doi: 10.1016/j.exphem.2014.05.007. Epub 2014 May 23.
8
Increased IL-6 secretion by aged human mesenchymal stromal cells disrupts hematopoietic stem and progenitor cells' homeostasis.衰老的人间充质基质细胞白细胞介素-6分泌增加会破坏造血干细胞和祖细胞的稳态。
Oncotarget. 2016 Mar 22;7(12):13285-96. doi: 10.18632/oncotarget.7690.
9
A novel 3D mesenchymal stem cell model of the multiple myeloma bone marrow niche: biologic and clinical applications.一种新型的多发性骨髓瘤骨髓微环境三维间充质干细胞模型：生物学及临床应用
Oncotarget. 2016 Nov 22;7(47):77326-77341. doi: 10.18632/oncotarget.12643.
10
Oncostatin M maintains the hematopoietic microenvironment in the bone marrow by modulating adipogenesis and osteogenesis.抑瘤素M通过调节脂肪生成和成骨作用维持骨髓中的造血微环境。
PLoS One. 2014 Dec 31;9(12):e116209. doi: 10.1371/journal.pone.0116209. eCollection 2014.

引用本文的文献

1
PNH clones prevalence study in ph-negative myeloproliferative neoplasms: a multicenter Italian study.阴性骨髓增殖性肿瘤中阵发性睡眠性血红蛋白尿克隆患病率研究：一项意大利多中心研究。
Ann Hematol. 2025 Aug 22. doi: 10.1007/s00277-025-06556-y.
2
Inflammatory signaling in the pathogenesis of acute myeloid leukemia.急性髓系白血病发病机制中的炎症信号传导
Hemasphere. 2025 Aug 13;9(8):e70188. doi: 10.1002/hem3.70188. eCollection 2025 Aug.
3
Mesenchymal stromal cells in myeloid malignancies: Immunotherapeutic opportunities.骨髓恶性肿瘤中的间充质基质细胞：免疫治疗机遇

本文引用的文献

1
HDAC8 overexpression in mesenchymal stromal cells from JAK2+ myeloproliferative neoplasms: a new therapeutic target?JAK2+骨髓增殖性肿瘤间充质基质细胞中HDAC8的过表达：一个新的治疗靶点？
Oncotarget. 2017 Apr 25;8(17):28187-28202. doi: 10.18632/oncotarget.15969.
2
A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells.CD90（THY-1）表达的降低导致间充质基质细胞分化增加。
Stem Cell Res Ther. 2016 Jul 28;7(1):97. doi: 10.1186/s13287-016-0359-3.
3
The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia.
Heliyon. 2024 Jan 22;10(3):e25081. doi: 10.1016/j.heliyon.2024.e25081. eCollection 2024 Feb 15.
4
Bone Marrow Niches and Tumour Cells: Lights and Shadows of a Mutual Relationship.骨髓龛与肿瘤细胞：相互关系的明暗面。
Front Immunol. 2022 May 6;13:884024. doi: 10.3389/fimmu.2022.884024. eCollection 2022.
5
The EHA Research Roadmap: Hematopoietic Stem Cells and Allotransplantation.欧洲血液学协会研究路线图：造血干细胞与同种异体移植
Hemasphere. 2022 Apr 29;6(5):e0714. doi: 10.1097/HS9.0000000000000714. eCollection 2022 May.
6
The EHA Research Roadmap: Normal Hematopoiesis.欧洲血液学协会研究路线图：正常造血
Hemasphere. 2021 Nov 30;5(12):e669. doi: 10.1097/HS9.0000000000000669. eCollection 2021 Dec.
7
NFĸB Targeting in Bone Marrow Mesenchymal Stem Cell-Mediated Support of Age-Linked Hematological Malignancies.靶向核因子κB在骨髓间充质干细胞介导的与年龄相关血液系统恶性肿瘤支持中的作用
Stem Cell Rev Rep. 2021 Dec;17(6):2178-2192. doi: 10.1007/s12015-021-10235-6. Epub 2021 Aug 19.
8
Myeloid-Derived Suppressor Cells and Mesenchymal Stem/Stromal Cells in Myeloid Malignancies.髓系恶性肿瘤中的髓源性抑制细胞和间充质干/基质细胞
J Clin Med. 2021 Jun 24;10(13):2788. doi: 10.3390/jcm10132788.
9
Inflammatory Microenvironment and Specific T Cells in Myeloproliferative Neoplasms: Immunopathogenesis and Novel Immunotherapies.骨髓增殖性肿瘤中的炎症微环境与特异性T细胞：免疫发病机制与新型免疫疗法。
Int J Mol Sci. 2021 Feb 14;22(4):1906. doi: 10.3390/ijms22041906.
10
A Broad Overview of Signaling in -Negative Classic Myeloproliferative Neoplasms.阴性经典型骨髓增殖性肿瘤中的信号传导概述
Cancers (Basel). 2021 Feb 26;13(5):984. doi: 10.3390/cancers13050984.
2016 年版世界卫生组织髓系肿瘤和急性白血病分类。
Blood. 2016 May 19;127(20):2391-405. doi: 10.1182/blood-2016-03-643544. Epub 2016 Apr 11.
4
Vav1 Regulates Mesenchymal Stem Cell Differentiation Decision Between Adipocyte and Chondrocyte via Sirt1.Vav1通过Sirt1调节间充质干细胞在脂肪细胞和软骨细胞之间的分化决定。
Stem Cells. 2016 Jul;34(7):1934-46. doi: 10.1002/stem.2365. Epub 2016 Apr 21.
5
Rationale for revision and proposed changes of the WHO diagnostic criteria for polycythemia vera, essential thrombocythemia and primary myelofibrosis.世界卫生组织真性红细胞增多症、原发性血小板增多症和原发性骨髓纤维化诊断标准的修订理由及拟议更改
Blood Cancer J. 2015 Aug 14;5(8):e337. doi: 10.1038/bcj.2015.64.
6
MSC surface markers (CD44, CD73, and CD90) can identify human MSC-derived extracellular vesicles by conventional flow cytometry.间充质干细胞表面标志物（CD44、CD73和CD90）可通过传统流式细胞术识别源自人间充质干细胞的细胞外囊泡。
Cell Commun Signal. 2016 Jan 12;14:2. doi: 10.1186/s12964-015-0124-8.
7
The Hematopoietic Niche in Myeloproliferative Neoplasms.骨髓增殖性肿瘤中的造血微环境。
Mediators Inflamm. 2015;2015:347270. doi: 10.1155/2015/347270. Epub 2015 Nov 30.
8
The Bone Marrow-Mediated Protection of Myeloproliferative Neoplastic Cells to Vorinostat and Ruxolitinib Relies on the Activation of JNK and PI3K Signalling Pathways.骨髓介导的骨髓增殖性肿瘤细胞对伏立诺他和鲁索替尼的保护作用依赖于JNK和PI3K信号通路的激活。
PLoS One. 2015 Dec 1;10(12):e0143897. doi: 10.1371/journal.pone.0143897. eCollection 2015.
9
Osteogenic Potential of Mesenchymal Stromal Cells Contributes to Primary Myelofibrosis.间质基质细胞的成骨潜能有助于原发性骨髓纤维化。
Cancer Res. 2015 Nov 15;75(22):4753-65. doi: 10.1158/0008-5472.CAN-14-3696. Epub 2015 Sep 24.
10
Normal and leukemic stem cell niches: insights and therapeutic opportunities.正常和白血病干细胞龛：见解与治疗机会。
Cell Stem Cell. 2015 Mar 5;16(3):254-67. doi: 10.1016/j.stem.2015.02.014.