Suh Y H, Park H Y, Lim J K, Kinemuchi H
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1986;85(2):401-7. doi: 10.1016/0742-8413(86)90216-1.
The effects of lipid-protein interactions on carp brain and liver mitochondrial MAO with respect to substrate and inhibitor preference, thermostability and Arrhenius parameters were studied and compared. Treatment with phospholipase A2, C or D decreased MAO activities towards 5-hydroxytryptamine (5-HT), beta-phenylethylamine and tyramine similarly, accompanied by great changes in their apparent affinities for MAO, but not by changes in Vmax values. Minimum phospholipid binding to mitochondria might be essential for enzyme activity. Among these activities, 5-HT deamination was the most sensitive to the changes in mitochondrial phospholipids and bulk lipid phase transition (fluidity). Sensitivity of MAO to clorgyline or l-deprenyl was not affected by these phospholipase treatments. Of the phospholipids tested, only phosphatidylinositol significantly activated MAO activity towards 5-HT in both intact and phospholipase-treated mitochondria.
研究并比较了脂蛋白相互作用对鲤鱼脑和肝线粒体单胺氧化酶（MAO）在底物和抑制剂偏好、热稳定性以及阿累尼乌斯参数方面的影响。用磷脂酶A2、C或D处理会类似地降低MAO对5-羟色胺（5-HT）、β-苯乙胺和酪胺的活性，同时伴随着它们对MAO的表观亲和力发生巨大变化，但Vmax值没有改变。线粒体与磷脂的最小结合可能对酶活性至关重要。在这些活性中，5-HT脱氨对线粒体磷脂和整体脂质相转变（流动性）的变化最为敏感。这些磷脂酶处理不影响MAO对氯吉兰或左旋司来吉兰的敏感性。在所测试的磷脂中，只有磷脂酰肌醇在完整和经磷脂酶处理的线粒体中均能显著激活MAO对5-HT的活性。
