Huang Chao-Min, Chang Kuo-Chin, Hung Chao-Hung, Chiu King-Wah, Lu Sheng-Nan, Wang Jing-Houng, Chen Chien-Hung, Kee Kwong-Ming, Kuo Yuan-Hung, Tsai Ming-Chao, Tseng Po-Lin, Lin Ming-Tsung, Wu Cheng-Kun, Hu Tsung-Hui, Cho Chung-Lung, Yen Yi-Hao
Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan.
Division of Hepato-Gastroenterology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
PLoS One. 2017 Aug 10;12(8):e0182204. doi: 10.1371/journal.pone.0182204. eCollection 2017.
A recent meta-analysis revealed that the genotype PNPLA3 rs738409 GG is associated with a higher risk of hepatic steatosis (HS) in Caucasian patients with chronic hepatitis C (CHC). However, controversial results were found regarding Asian populations. Furthermore, previous studies have shown a negative association between interferon lambda 3 (IFNL3) rs12979860 CC and HS in Caucasian CHC patients, but there have been no reports indicating any such association in Asian populations. In this study, then, we investigated the association of PNPLA3 and IFNL3 polymorphisms with HS in Asian CHC patients.
We enrolled consecutive CHC patients who underwent liver biopsy prior to antiviral therapy. We excluded those patients with decompensated liver disease, any co-existing chronic liver disease, or HIV or HBV co-infection.
1080 CHC patients were enrolled, and HS was found in 453 (41.9%) patients. The frequency distribution of the G allele was significantly associated with HS (P<0.001), and this conferred a higher risk to G allele homozygotes (OR: 2.06, 95% CI: 1.46-2.88, P <0.001) than to G allele carriers (OR: 1.98, 95% CI: 1.52-2.58, P<0.001). There was a borderline significant difference in the prevalence of HS in rs12979860 CC versus non-CC (40.8% versus 49.3%, P = 0.059). After adjustment for age, sex, body mass index, diabetes, and excessive alcohol intake, the rs738409 G allele homozygote carriers still carried a higher risk for HS (OR: 1.93, 95% CI: 1.35-2.77, P = 0.003).
The PNPLA3 rs738409 GG genotype is positively associated with HS, while the IFNL3 rs 12979860 CC genotype may be negatively associated with HS, in Asian CHC patients.
最近一项荟萃分析显示，在患有慢性丙型肝炎（CHC）的白种人患者中，PNPLA3基因rs738409位点的GG基因型与肝脂肪变性（HS）风险较高相关。然而，在亚洲人群中发现了相互矛盾的结果。此外，先前的研究表明，在白种人CHC患者中，干扰素λ3（IFNL3）基因rs12979860位点的CC基因型与HS呈负相关，但尚无报告表明在亚洲人群中存在此类关联。因此，在本研究中，我们调查了亚洲CHC患者中PNPLA3和IFNL3基因多态性与HS的关联。
我们纳入了在接受抗病毒治疗前接受肝活检的连续性CHC患者。我们排除了那些患有失代偿性肝病、任何并存的慢性肝病或合并HIV或HBV感染的患者。
共纳入1080例CHC患者，其中453例（41.9%）患者存在HS。G等位基因的频率分布与HS显著相关（P<0.001），与G等位基因携带者相比，G等位基因纯合子发生HS的风险更高（OR：2.06，95%CI：1.46-2.88，P<0.001）（OR：1.98，95%CI：1.52-2.58，P<0.001）。rs12979860位点CC基因型与非CC基因型的HS患病率存在临界显著差异（40.8%对49.3%，P = 0.059）。在调整年龄、性别、体重指数、糖尿病和过量饮酒因素后，rs738409位点G等位基因纯合子携带者发生HS的风险仍然较高（OR：1.93，95%CI：1.35-2.77，P = 0.003）。
在亚洲CHC患者中，PNPLA3基因rs738409位点的GG基因型与HS呈正相关，而IFNL3基因rs12979860位点的CC基因型可能与HS呈负相关。
