人类HRAS1癌基因VTR区域中两种TaqI多态性的检测。
Detection of two TaqI polymorphisms in the VTR region of the human HRAS1 oncogene.
作者信息
Pierotti M A, Radice P, Biunno I, Borrello M G, Cattadori M R, Della Porta G
出版信息
Cytogenet Cell Genet. 1986;43(3-4):174-80. doi: 10.1159/000132317.
Restriction enzyme analysis of the variable tandem repetition (VTR) region of the human HRAS1 oncogene revealed two TaqI polymorphisms. The first polymorphism overlaps that detected with MspI/HpaII restriction enzymes and is due to a variable number of repeat units which form the VTR region at the 3' end of the oncogene. The second polymorphism appears to be due to two new TaqI restriction sites created within the VTR region itself. This novel polymorphism was detected in 26 of 145 human DNA samples and was found to segregate in a Mendelian fashion.
对人类HRAS1癌基因可变串联重复（VTR）区域进行的限制性内切酶分析揭示了两种TaqI多态性。第一种多态性与用MspI/HpaII限制性内切酶检测到的多态性重叠，它是由于构成癌基因3'端VTR区域的重复单元数量可变所致。第二种多态性似乎是由于在VTR区域自身内产生的两个新的TaqI限制性酶切位点所致。在145份人类DNA样本中的26份中检测到了这种新的多态性，并且发现它以孟德尔方式分离。
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