Machado Ana Paula, Pannell John R, Tonnabel Jeanne
Department of Ecology and Evolution, Biophore Building, University of Lausanne, 1015, Lausanne, Switzerland.
BMC Res Notes. 2017 Aug 10;10(1):386. doi: 10.1186/s13104-017-2700-z.
Mercurialis annua is a wind-pollinated annual plant that has long been used as a model for the study of ploidy and sexual-systems evolution. However, no molecular markers are yet available for genetic studies of its diploid populations. Here, we develop and characterize a set of eight polymorphic microsatellite markers for diploid dioecious M. annua.
Following an SSR-enrichment protocol, 13 microsatellite markers were proposed, eight of which yielded successful amplification and polymorphism. We screened the eight microsatellite loci in 100 individuals. The number of alleles per marker ranged from 6 to 12, and observed heterozygosity ranged from 0.57 to 0.76. To estimate potential allele scoring errors, these individuals' offspring were genotyped for the same loci, and error rates were estimated from parentage analyses. Error rates ranged from 0 to 6.8%. Cross-amplification tests were performed for congeneric M. huetti and M. canariensis, with successful amplification for seven and six of the eight loci, respectively. The novel microsatellite markers proposed here will be crucial for a multitude of genetic studies of M. annua and further establish its importance as a model species for addressing ecological and population genetic questions.
一年生山靛是一种风媒传粉的一年生植物，长期以来一直被用作研究倍性和性系统进化的模型。然而，目前尚无用于其二倍体种群遗传研究的分子标记。在此，我们开发并鉴定了一组针对二倍体雌雄异株一年生山靛的八个多态性微卫星标记。
按照SSR富集方案，提出了13个微卫星标记，其中8个成功扩增并具有多态性。我们在100个个体中筛选了这8个微卫星位点。每个标记的等位基因数量在6到12之间，观察到的杂合度在0.57到0.76之间。为了估计潜在的等位基因评分误差，对这些个体的后代进行了相同位点的基因分型，并通过亲权分析估计误差率。误差率在0到6.8%之间。对同属的休氏山靛和加那利山靛进行了交叉扩增测试，8个位点中分别有7个和6个成功扩增。这里提出的新型微卫星标记对于一年生山靛的大量遗传研究至关重要，并进一步确立了其作为解决生态和种群遗传问题的模式物种的重要性。
