Clayman Carly L, Malloy Elizabeth J, Kearns David N, Connaughton Victoria P
Department of Psychology, American University, Washington, DC, 20016, USA; Center for Behavioral Neuroscience, American University, Washington, DC, 20016, USA.
Department of Mathematics, American University, Washington, DC 20016, USA.
Behav Brain Res. 2017 Sep 29;335:174-184. doi: 10.1016/j.bbr.2017.08.007. Epub 2017 Aug 8.
Alcohol exposure in adolescence is a contributing factor toward reward-seeking behavior in adulthood. This reward-seeking behavior is assessed in animal models using the conditioned place preference (CPP) paradigm. In this study, ethanol-induced change in time spent by zebrafish on the initially non-preferred tank side was studied by conditioning adult zebrafish to ethanol dissolved in water (0.00% 1.00%; 1.25%; 1.50%; 1.60%; 1.75% vol/vol) paired with an initially non-preferred environment. Following a single conditioning cycle, fish swam unrestricted in the CPP chamber to assess changes in preference. Daily 20-min pre-exposure to ethanol for 1 week during the juvenile stage starting at either 20days post fertilization (dpf) or 40 dpf altered percent time spent on the ethanol-paired side in adulthood in a dose-dependent and sex-dependent manner. The results suggest that male and female zebrafish are an effective model in which to investigate behavioral correlates of ethanol-induced changes in neural circuits implicated in reward and anxiety.
青春期接触酒精是成年后寻求奖励行为的一个促成因素。这种寻求奖励的行为在动物模型中使用条件性位置偏好（CPP）范式进行评估。在本研究中，通过将成年斑马鱼与溶解在水中的乙醇（0.00%、1.00%、1.25%、1.50%、1.60%、1.75%体积/体积）配对，并置于最初不喜欢的环境中，研究了乙醇诱导斑马鱼在最初不喜欢的水箱一侧停留时间的变化。经过一个单一的条件循环后，鱼在CPP实验箱中自由游动，以评估偏好的变化。在幼鱼阶段，从受精后20天（dpf）或40 dpf开始，每天对乙醇进行20分钟的预暴露，持续1周，成年后在与乙醇配对一侧停留的时间百分比会以剂量和性别依赖的方式发生改变。结果表明，雄性和雌性斑马鱼是研究乙醇诱导的与奖励和焦虑相关的神经回路变化的行为相关性的有效模型。
