Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, China.
Platform of Bioethanol, Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, China; Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, China; Key Laboratory of Fermentation (Ministry of Education), Hubei University of Technology, Wuhan, China.
J Biotechnol. 2017 Oct 10;259:73-82. doi: 10.1016/j.jbiotec.2017.08.004. Epub 2017 Aug 8.
Sugarcane bagasse (SCB) is an abundant, renewable and inexpensive agricultural byproduct for the production of biofuel and other biobased products. To effectively saccharify SCB with cellulases, combination with dilute alkali salts NaSO/NaPO (0.4% NaPO, 0.03% NaSO) at 7.5% sulfidity and hot water (DASHW) in "one-pot" pretreatment media by autoclaving at 110°C for 40min was attempted to pretreat SCB in this study. Furthermore, FT-IR, XRD and SEM were employed to characterize the changes in the cellulose structural characteristics (porosity, morphology, and crystallinity) of the pretreated NaSO/NaPO-SCB solid residue, which indicated that combination pretreatment could effectively remove lignin and hemicellulose for enhancing enzymatic saccharification. After 72h, the reducing sugars and glucose from the enzymatic in situ hydrolysis of 50g/L NaSO/NaPO-SCB in dilute NaSO/NaPO (0.27% NaPO, 0.02% NaSO) media were obtained at 33.8 and 21.8g/L, respectively. Finally, the SCB-hydrolysates containing 20g/L glucose were used for ethanol fermentation in the presence of dilute alkali salts. After 48h, the ethanol yield was 0.42g ethanol/g glucose, which represents 82.1% of the theoretical yield. In conclusion, this study provided an effective pretreatment strategy for enhancing SCB's saccharification, which has potential application of other lignocellulosic materials.
甘蔗渣 (SCB) 是一种丰富、可再生且廉价的农业副产物，可用于生产生物燃料和其他生物基产品。为了有效地用纤维素酶糖化 SCB，本研究尝试在 110°C 下用高压锅蒸煮 40min，将 0.4% 的 NaPO 和 0.03% 的 NaSO 的 NaSO/NaPO（7.5% 的硫化度）与热水（DASHW）组合在“一锅”预处理介质中预处理 SCB。此外，采用傅里叶变换红外光谱 (FT-IR)、X 射线衍射 (XRD) 和扫描电子显微镜 (SEM) 对预处理 NaSO/NaPO-SCB 固体残渣中纤维素结构特性（孔隙率、形态和结晶度）的变化进行了表征，结果表明组合预处理可以有效去除木质素和半纤维素，从而增强酶糖化作用。72h 后，在 0.27% 的 NaPO 和 0.02% 的 NaSO 的稀 NaSO/NaPO 介质中，通过酶原位水解 50g/L 的 NaSO/NaPO-SCB 可获得 33.8g/L 的还原糖和 21.8g/L 的葡萄糖。最后，在稀碱盐存在的情况下，用 20g/L 的葡萄糖对 SCB 水解液进行乙醇发酵。48h 后，乙醇得率为 0.42g 乙醇/g 葡萄糖，为理论产率的 82.1%。总之，本研究为提高 SCB 的糖化效率提供了一种有效的预处理策略，该策略可能适用于其他木质纤维素材料。
