Veloo A C M, Jean-Pierre H, Justesen U S, Morris T, Urban E, Wybo I, Shah H N, Friedrich A W, Morris T, Shah H N, Jean-Pierre H, Justesen U S, Nagy E, Urban E, Kostrzewa M, Veloo A, Friedrich A W
Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, The Netherlands.
Centre Hospitalier Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France; Université Montpellier 1, UMR5119 ECOSYM, Equipe Pathogènes Hydriques Santé Environnements, UMR 5569 Hydrosciences, UFR Pharmacie, 15 avenue Charles Flahault, 34093 Montpellier Cedex 5, France.
Anaerobe. 2017 Dec;48:94-97. doi: 10.1016/j.anaerobe.2017.07.004. Epub 2017 Aug 7.
Inter-laboratory reproducibility of Matrix Assisted Laser Desorption Time-of-Flight Mass Spectrometry (MALDI-TOF MS) of anaerobic bacteria has not been shown before. Therefore, ten anonymized anaerobic strains were sent to seven participating laboratories, an initiative of the European Network for the Rapid Identification of Anaerobes (ENRIA). On arrival the strains were cultured and identified using MALDI-TOF MS. The spectra derived were compared with two different Biotyper MALDI-TOF MS databases, the db5627 and the db6903. The results obtained using the db5627 shows a reasonable variation between the different laboratories. However, when a more optimized database is used, the variation is less pronounced. In this study we show that an optimized database not only results in a higher number of strains which can be identified using MALDI-TOF MS, but also corrects for differences in performance between laboratories.
此前尚未证明厌氧细菌的基质辅助激光解吸飞行时间质谱（MALDI-TOF MS）在不同实验室间的可重复性。因此，十株匿名厌氧菌株被送至七个参与实验室，这是欧洲厌氧菌快速鉴定网络（ENRIA）发起的一项活动。菌株送达后，使用MALDI-TOF MS进行培养和鉴定。所得光谱与两个不同的Biotyper MALDI-TOF MS数据库db5627和db6903进行比较。使用db5627获得的结果显示不同实验室之间存在合理差异。然而，当使用更优化的数据库时，差异就不那么明显了。在本研究中，我们表明优化后的数据库不仅能使更多菌株通过MALDI-TOF MS得到鉴定，还能校正不同实验室之间的性能差异。
