Qian Jin, Wei Li, Wu Yaoguo, Wang Qilin, Fu Xiaoying, Zhang Xiaochao, Chang Xing, Wang Lianlian, Pei Xiangjun
Department of Applied Chemistry, School of Natural and Applied Sciences, Research & Development Institute in Shenzhen, Northwestern Polytechnical University, China; State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610065, China; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu University of Technology, Chengdu, 610059, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China.
Chemosphere. 2017 Nov;186:322-330. doi: 10.1016/j.chemosphere.2017.07.106. Epub 2017 Jul 19.
A comparative study on denitrifying sludge granulation with different electron donors (sulfide, thiosulfate and organics) was carried out. Longer time was spent on sulfide-denitrifying granular sludge (DGS) cultivation (88 days) than thiosulfate- and organics-DGS cultivations (57 days). All the three DGS were characterized in terms of particle size distribution, sludge settling ability (indicated by sludge volume index and settling velocity), permeability (indicated by fractal dimension) and extracellular polymeric substances (EPS, including polysaccharide and protein) secretion. Sludge productions in the three DGS-reactors were also monitored. The key functional microorganisms in three granular reactors were revealed via high through-put pyrosequencing analysis. Batch tests were performed to measure the denitrification activities of each DGS, including both denitratation (NO → NO) and denitritation (NO → N). We found that thiosulfate-driven denitrifying sludge granulation (TDDSG) should be the most efficient and compact technology for effective BNR in municipal wastewater treatment. The findings of this study suggests the TDDSG could further increase the nitrogen removal potential in an enhanced sulfur cycle-driven bioprocess for co-treatment of wet flue gas desulfurization wastes with fresh sewage depending on three short-cut biological reactions, including: 1) short-cut biological sulfur reduction (SO/SO → SO); 2) thiosulfate-driven denitritation (SO + NO → SO + N↑); and 3) nitritation (NH + O → NO).
开展了一项关于不同电子供体（硫化物、硫代硫酸盐和有机物）反硝化污泥颗粒化的比较研究。硫化物反硝化颗粒污泥（DGS）培养耗时较长（88天），而硫代硫酸盐和有机物DGS培养耗时较短（57天）。对所有三种DGS进行了粒度分布、污泥沉降能力（用污泥体积指数和沉降速度表示）、渗透性（用分形维数表示）和胞外聚合物（EPS，包括多糖和蛋白质）分泌特性表征。还监测了三个DGS反应器中的污泥产量。通过高通量焦磷酸测序分析揭示了三个颗粒反应器中的关键功能微生物。进行了批次试验以测量每种DGS的反硝化活性，包括脱硝（NO → NO）和脱亚硝酸盐（NO → N）。我们发现，硫代硫酸盐驱动的反硝化污泥颗粒化（TDDSG）应该是城市污水处理中有效生物脱氮除磷最有效且紧凑的技术。本研究结果表明，TDDSG可在强化硫循环驱动的生物工艺中进一步提高氮去除潜力，该工艺用于协同处理湿法烟气脱硫废物与新鲜污水，这取决于三个短程生物反应，包括：1）短程生物硫还原（SO/SO → SO）；2）硫代硫酸盐驱动的脱亚硝酸盐反应（SO + NO → SO + N↑）；3）亚硝化反应（NH + O → NO）。
