Characterization of Macrophage Polarization States Using Combined Measurement of 2-Deoxyglucose and Glutamine Accumulation: Implications for Imaging of Atherosclerosis.

OBJECTIVE

Despite the early promising results of F-fluorodeoxyglucose positron emission tomography for assessment of vessel wall inflammation, its accuracy in prospective identification of vulnerable plaques has remained limited. Additionally, previous studies have indicated that F-fluorodeoxyglucose uptake alone may not allow for accurate identification of specific macrophage activation states. We aimed to determine whether combined measurement of glucose and glutamine accumulation-the 2 most important bioenergetic substrates for macrophages-improves the distinction of macrophage inflammatory states and can be utilized to image atherosclerosis.

APPROACH AND RESULTS

Murine peritoneal macrophages (MΦ) were activated ex vivo into proinflammatory states with either lipopolysaccharide (MΦ) or interferon-γ+tumor necrosis factor-α (MΦ). An alternative polarization phenotype was induced with interleukin-4 (MΦ). The pronounced increase in 2-deoxyglucose uptake distinguishes MΦ from MΦ, MΦ, and unstimulated macrophages (MΦ). Despite having comparable levels of 2-deoxyglucose accumulation, MΦ can be distinguished from both MΦ and MΦ based on the enhanced glutamine accumulation, which was associated with increased expression of a glutamine transporter, . Ex vivo autoradiography experiments demonstrated distinct and heterogenous patterns of F-fluorodeoxyglucose and C-glutamine accumulation in atherosclerotic lesions of low-density lipoprotein receptor-null mice fed a high-fat diet.

CONCLUSIONS

Combined assessment of glutamine and 2-deoxyglucose accumulation improves the ex vivo identification of macrophage activation states. Combined ex vivo metabolic imaging demonstrates heterogenous and distinct patterns of substrate accumulation in atherosclerotic lesions. Further studies are required to define the in vivo significance of glutamine uptake in atherosclerosis and its potential application in identification of vulnerable plaques.