Schreiber Catharina, Eilenberg Magdalena, Kiss Attila, Bergmeister Helga, Podesser Bruno, Mascherbauer Julia, Bonderman Diana
Department of Internal Medicine II, Division of Cardiology, Medical University of Vienna, Vienna, Austria.
Clinic for Cardiac Surgery, Paracelsus Medical University Salzburg, Salzburg, Austria.
Am J Physiol Lung Cell Mol Physiol. 2017 Nov 1;313(5):L957-L963. doi: 10.1152/ajplung.00278.2017. Epub 2017 Aug 10.
Telemetric monitoring of hemodynamic parameters has become an established standard in experimental models of pulmonary arterial hypertension (PAH). To that purpose, a dedicated catheter is usually implanted through the right ventricular wall of study animals. Drawbacks of this standard technique are as follows: obtained pressures are from the right ventricle and therefore only surrogates for pulmonary arterial pressures, and furthermore, right ventricular myocardium is always damaged to a certain degree. To overcome shortcomings of standard hemodynamic assessment, we modified an established rat model, where severe PAH is induced by left-sided pneumonectomy plus monocrotaline injection. We describe here a novel telemetry catheter implantation technique, where the device is advanced into the pulmonary artery via the remaining stump and the transmitter is placed in a subcutaneous pocket. A total of 105 rats were operated with a median (range) implantation time of 50 (30-88) min and an excellent perioperative survival of 93%. After monocrotaline induction on , animals developed severe PAH with mean ± SD pressures of 75.9 ± 18.6 (systolic), 55.0 ± 18.0 (mean), and 42.1 ± 21.3 mmHg (diastolic) after 4 wk. Postmortem, the animals showed severe right ventricular hypertrophy, and histological analysis confirmed excessive medial hypertrophy and intimal hyperplasia, both characteristic features of human PAH. Comparison of the new telemetric model with standard microtip catheterization did not show relevant measurement differences. We established the first experimental animal model for PAH with preserved right ventricular integrity that allows direct telemetric monitoring of real-time systolic, mean, and diastolic pressures in the main pulmonary artery of freely moving rats.
血流动力学参数的遥测监测已成为肺动脉高压（PAH）实验模型中的既定标准。为此，通常通过研究动物的右心室壁植入专用导管。这种标准技术的缺点如下：所获得的压力来自右心室，因此仅是肺动脉压力的替代指标，而且，右心室心肌总会受到一定程度的损伤。为克服标准血流动力学评估的缺点，我们对已建立的大鼠模型进行了改良，在该模型中，通过左侧肺切除术加注射野百合碱诱导严重PAH。我们在此描述一种新颖的遥测导管植入技术，该装置通过剩余残端推进至肺动脉，且发射器置于皮下袋中。总共105只大鼠接受了手术，植入时间中位数（范围）为50（30 - 88）分钟，围手术期存活率高达93%。在注射野百合碱诱导后，4周时动物出现严重PAH，收缩压、平均压和舒张压的均值±标准差分别为75.9 ± 18.6 mmHg、55.0 ± 18.0 mmHg和42.1 ± 21.3 mmHg。死后检查发现，动物出现严重的右心室肥厚，组织学分析证实存在过度的中膜肥厚和内膜增生，这两者都是人类PAH的特征性表现。新的遥测模型与标准微尖端导管插入术的比较未显示出相关测量差异。我们建立了首个右心室完整性得以保留的PAH实验动物模型，该模型能够对自由活动大鼠的主肺动脉实时收缩压、平均压和舒张压进行直接遥测监测。
