Molecular Basis Underlying Leaf Variegation of a Moth Orchid Mutant ( subsp. ).

Leaf variegation is often the focus of plant breeding. Here, we studied a variegated mutant of subsp. , which is usually used as a parent of horticultural breeding, to understand its anatomic and genetic regulatory mechanisms in variegation. Chloroplasts with well-organized thylakoids and starch grains were found only in the mesophyll cells of green sectors but not of yellow sectors, confirming that the variegation belongs to the chlorophyll type. The two-dimensional electrophoresis and LC/MS/MS also reveal differential expressions of PsbP and PsbO between the green and yellow leaf sectors. Full-length cDNA sequencing revealed that mutant transcripts were caused by intron retention. When conditioning on the total RNA expression, we found that the functional transcript of and mutant transcript of are higher expressed in the yellow sector than in the green sector, suggesting that the post-transcriptional regulation of and differentiates the performance between green and yellow sectors. Because plays an important role in the stability of thylakoid folding, we suggest that the negative regulation of may inhibit thylakoid development in the yellow sectors. This causes chlorophyll deficiency in the yellow sectors and results in leaf variegation. We also provide evidence of the link of virus CymMV and the formation of variegation according to the differential expression of CymMV between green and yellow sectors.