Yang Jing, Zhang Li, Wang Handong, Guo Zan, Liu Yixian, Zhang Yi, Wang Chuan, Li Quanhai
Department of Physiology, Hebei Medical University, Shijiazhuang, Hebei 050017, China.
Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang, Hebei 050017, China.
Stem Cells Int. 2017;2017:5706193. doi: 10.1155/2017/5706193. Epub 2017 Jul 16.
Aplastic anemia (AA) is a common malignant blood disease, and chronic intermittent hypobaric hypoxia (CIHH) has a beneficial effect against different diseases. The aim of the present study was to investigate the protective effect of CIHH against AA and underlying mechanisms. 5-Fluorouracil and busulfan treatment induced AA model in rats with reduction of hematological parameters and bone marrow tissue injury and decrease of the colony numbers of progenitor cells. CIHH pretreatment significantly reduced the incidence rate of AA and alleviated above symptoms in AA model. The adhesive molecules of bone marrow mesenchymal stem cells (BMMSCs) in AA model, VLA-4, VCAM-1, and ICAM-1 were upregulated, and those of CD162 and CD164 were downregulated by CIHH pretreatment. The expressions of HIF-1 and NF-B in BMMSCs were also decreased through CIHH pretreatment. Overall, the results demonstrated for the first time that CIHH has an anti-AA effect through improving the adhesiveness and stress of mesenchymal stem cells in rats. CIHH could be a promising and effective therapy for AA.
再生障碍性贫血（AA）是一种常见的恶性血液疾病，而慢性间歇性低压缺氧（CIHH）对不同疾病具有有益作用。本研究的目的是探讨CIHH对AA的保护作用及其潜在机制。5-氟尿嘧啶和白消安处理诱导大鼠AA模型，导致血液学参数降低、骨髓组织损伤以及祖细胞集落数量减少。CIHH预处理显著降低了AA模型的发病率，并减轻了上述症状。CIHH预处理使AA模型中骨髓间充质干细胞（BMMSCs）的黏附分子VLA-4、VCAM-1和ICAM-1上调，而CD162和CD164下调。CIHH预处理还降低了BMMSCs中HIF-1和NF-κB的表达。总体而言，结果首次证明CIHH通过改善大鼠间充质干细胞的黏附性和应激性而具有抗AA作用。CIHH可能是一种有前景且有效的AA治疗方法。
