Kim Haebeen, Park Hae-Ryoun, Song Jae Min, Yeom Eunseop
School of Mechanical Engineering, Pusan National University, Busan, South Korea.
Department of Oral Pathology, School of Dentistry, Pusan National University, Yangsan, South Korea.
Biomicrofluidics. 2025 Jan 3;19(1):014101. doi: 10.1063/5.0237452. eCollection 2025 Jan.
Monitoring platelet aggregation is crucial for predicting thrombotic diseases and identifying the risk of bleeding or resistance to antiplatelet drugs. This study developed a microfluidic device to measure platelet activation with high sensitivity. By controlling exposure time through repeated reinjections, the device enables the detection of subtle changes in platelet activity influenced by lifestyle factors, such as alcohol consumption. Using computational fluid dynamics simulations, the design was optimized to achieve moderate shear stresses and fabricated with 3D printing. Experimental results revealed that pillars biased to one side partially accelerate the flow and inhibit platelet adhesion. A distinct difference in platelet adhesion was clearly observed before and after alcohol consumption. Despite the high standard deviations in platelet adhesion area, hematocrit, and viscosity after alcohol consumption, the area covered by adhered platelets increased by 3.12 times compared to that before alcohol consumption. This microfluidic chip offers potential for personalized health monitoring by distinguishing platelet variations caused by lifestyle or dietary habits. However, challenges such as reinjection procedures and large sample volumes require further investigation.
监测血小板聚集对于预测血栓性疾病以及识别出血风险或抗血小板药物抵抗性至关重要。本研究开发了一种微流控装置，用于高灵敏度地测量血小板活化。通过重复再注射来控制暴露时间，该装置能够检测受生活方式因素（如饮酒）影响的血小板活性的细微变化。利用计算流体动力学模拟，对设计进行了优化，以实现适度的剪切应力，并通过3D打印制造。实验结果表明，向一侧倾斜的支柱会部分加速流动并抑制血小板粘附。饮酒前后明显观察到血小板粘附存在显著差异。尽管饮酒后血小板粘附面积、血细胞比容和粘度的标准偏差较高，但与饮酒前相比，粘附血小板覆盖的面积增加了3.12倍。这种微流控芯片通过区分由生活方式或饮食习惯引起的血小板变化，为个性化健康监测提供了潜力。然而，再注射程序和大样本量等挑战需要进一步研究。
