Univ. Grenoble Alpes , Centre de Recherches sur les Macromolécules Végétales (CERMAV), F-38000 Grenoble, France.
CNRS, CERMAV , F-38000 Grenoble, France.
Biomacromolecules. 2017 Sep 11;18(9):2918-2927. doi: 10.1021/acs.biomac.7b00872. Epub 2017 Aug 29.
The interaction of 1,2 dioleolyl-sn-glycero-3-phosphatidylcholine (DOPC) vesicles with cellulose nanocrystals (CNCs) using several complementary techniques. Dynamic light scattering, zeta-potential, cryo-transmission electron microscopy and isothermal titration calorimetry (ITC) analyses confirmed the formation of pH-dependent CNC-liposome complexes. ITC was used to characterize the thermodynamic properties of this interaction. Positive values of enthalpy were found at pH lower than 5 where the charge sign of the constituents was opposite. The association was more pronounced at lower pH, as indicated by the higher values of association constant. We suggest that the positive enthalpy is derived from the release of counterions from the particle hydration shell during the association and that the charge of the vesicles plays a significant role in this interaction.
使用几种互补技术研究 1,2 二油酰基-sn-甘油-3-磷酸胆碱（DOPC）囊泡与纤维素纳米晶体（CNC）的相互作用。动态光散射、zeta 电位、冷冻传输电子显微镜和等温热力学滴定（ITC）分析证实了 pH 依赖性 CNC-脂质体复合物的形成。ITC 用于表征这种相互作用的热力学性质。在低于 5 的 pH 值下，当组分的电荷符号相反时，发现焓值为正值。在较低 pH 值下，由于结合常数的值更高，因此结合更明显。我们认为正焓值源自于在结合过程中从粒子水合壳中释放抗衡离子，并且囊泡的电荷在这种相互作用中起着重要作用。
