Ghosh Raja, Chen Guoqiang
Department of Chemical Engineering, McMaster University, 1280 Main Street West Hamilton, Ontario, L8S 4L7, Canada.
Department of Chemical Engineering, McMaster University, 1280 Main Street West Hamilton, Ontario, L8S 4L7, Canada.
J Chromatogr A. 2017 Sep 15;1515:138-145. doi: 10.1016/j.chroma.2017.07.088. Epub 2017 Jul 31.
In a recent paper, box-shaped or cuboid packed bed devices have been proposed as alternative to columns for chromatographic separations. We first propose a mathematical model for residence time distribution in such devices. Based on it, we examine factors likely to affect separation performance, and verify the predictions of our mathematical model by conducting tracer experiments. We then compare the performance of two commercial columns with their respective equivalent cuboid packed-bed devices, i.e. containing the same chromatographic media, and having the same bed-height and bed-volume. Parameters compared include the number of theoretical plates, attributes of flow-through and eluted protein peaks, and resolution in model binary protein separations. For each of these metrics examined, the cuboid packed-bed device outperformed its equivalent commercial column. Other potential advantages likely to be gained from using a cuboid packed-bed instead of a column are elucidated.
在最近的一篇论文中，已提出将箱形或长方体填充床设备用作色谱分离柱的替代方案。我们首先提出了此类设备中停留时间分布的数学模型。基于该模型，我们研究了可能影响分离性能的因素，并通过进行示踪剂实验验证了数学模型的预测结果。然后，我们将两根商用色谱柱的性能与其各自等效的长方体填充床设备进行比较，即这些设备含有相同的色谱介质，且具有相同的床高和床体积。比较的参数包括理论塔板数、流过和洗脱蛋白质峰的属性，以及模型二元蛋白质分离中的分辨率。对于所考察的这些指标中的每一项，长方体填充床设备的性能均优于其等效的商用色谱柱。文中还阐明了使用长方体填充床而非色谱柱可能获得的其他潜在优势。
