[卵巢癌患者外周血中Th1/Th2与Treg/Th17细胞平衡]
[Th1/Th2 and Treg/Th17 cell balance in peripheral blood of patients with ovarian cancer].
作者信息
Wang Li-Hua, Wang Liang-Liang, Zhang Jing, Zhang Pei, Li Sheng-Ze
机构信息
Department of Gynecology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China. E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2017 Aug 20;37(8):1066-1070. doi: 10.3969/j.issn.1673-4254.2017.08.11.
OBJECTIVE
To investigate the changes in the percentages and balance of CD4T cell subsets including T helper cells (Thl, Th2, and Thl7) and T regulatory cells (Treg) in patients with ovarian cancer.
METHODS
Peripheral blood samples were collected from 30 patients with ovarian cancer and 20 healthy subjects for analysis of the percentages of Thl, Th2, Thl7 and Treg using flow cytometry.
RESULTS
Compared with the control subjects, the patients with ovarian cancer showed significantly increased percentages of Th2, Thl7 and Treg (P<0.05) but significantly decreased percentage of Th1 in the peripheral blood of patients with ovarian cancer (P<0.05). The changes in CD4 T cell subsets were significantly correlated with the clinical stage of the tumor (P<0.05) but not with the histological type or cell differentiation (P>0.05). The Th1/Th2 ratio was significantly decreased in ovarian cancer patients (P<0.05) with obvious Th2 polarization compared with control group. The Treg/Th17 ratio was significantly increased in ovarian cancer patients (P<0.05).
CONCLUSION
Patients with in ovarian cancer have abnormal expressions of CD4T cell subsets in the peripheral blood with Th1/Th2 and Treg/Th17 imbalance, and these findings provide evidence for clinical immunotherapy of ovarian cancer.
目的
探讨卵巢癌患者外周血中辅助性T细胞（Th1、Th2和Th17）及调节性T细胞（Treg）等CD4T细胞亚群百分比及平衡的变化。
方法
收集30例卵巢癌患者及20例健康对照者的外周血样本，采用流式细胞术分析Th1、Th2、Th17及Treg的百分比。
结果
与健康对照者相比，卵巢癌患者外周血中Th2、Th17及Treg的百分比显著升高（P<0.05），而Th1的百分比显著降低（P<0.05）。CD4T细胞亚群的变化与肿瘤临床分期显著相关（P<0.05），与组织学类型及细胞分化无关（P>0.05）。卵巢癌患者Th1/Th2比值显著降低（P<0.05），与对照组相比Th2极化明显。卵巢癌患者Treg/Th17比值显著升高（P<0.05）。
结论
卵巢癌患者外周血CD4T细胞亚群表达异常，Th1/Th2及Treg/Th17失衡，这些结果为卵巢癌临床免疫治疗提供了依据。
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