Hörner Maximilian, Müller Konrad, Weber Wilfried
Faculty of Biology, University of Freiburg, Freiburg, Germany.
Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany.
Methods Mol Biol. 2017;1651:173-186. doi: 10.1007/978-1-4939-7223-4_13.
Recent advances in the development of light-inducible transgene expression systems have overcome many inherent drawbacks of conventional chemically regulated systems. The latest generation of those light-regulated systems that are specifically responsive to different wavelengths allows spatiotemporal control of gene expression in a so far unprecedented manner.In this chapter, we first describe the available light-inducible gene expression systems compatible with mammalian cells and explain their underlying mechanisms. Afterward, we give a detailed protocol for the implementation of a UVB light-inducible expression system in mammalian cells.
光诱导转基因表达系统开发方面的最新进展克服了传统化学调控系统的许多固有缺点。最新一代对不同波长具有特异性响应的光调控系统能够以前所未有的方式对基因表达进行时空控制。在本章中，我们首先描述与哺乳动物细胞兼容的现有光诱导基因表达系统，并解释其潜在机制。之后，我们给出在哺乳动物细胞中实施UVB光诱导表达系统的详细方案。
