Müller Konrad, Naumann Sebastian, Weber Wilfried, Zurbriggen Matias D
Biol Chem. 2015 Feb;396(2):145-52. doi: 10.1515/hsz-2014-0199.
Molecular switches that are controlled by chemicals have evolved as central research instruments in mammalian cell biology. However, these tools are limited in terms of their spatiotemporal resolution due to freely diffusing inducers. These limitations have recently been addressed by the development of optogenetic, genetically encoded, and light-responsive tools that can be controlled with the unprecedented spatiotemporal precision of light. In this article, we first provide a brief overview of currently available optogenetic tools that have been designed to control diverse cellular processes. Then, we focus on recent developments in light-controlled gene expression technologies and provide the reader with a guideline for choosing the most suitable gene expression system.
受化学物质控制的分子开关已发展成为哺乳动物细胞生物学中的核心研究工具。然而，由于诱导剂的自由扩散，这些工具在时空分辨率方面存在局限性。最近，光遗传学、基因编码和光响应工具的发展解决了这些限制，这些工具可以以前所未有的光时空精度进行控制。在本文中，我们首先简要概述了目前可用的旨在控制各种细胞过程的光遗传学工具。然后，我们重点介绍光控基因表达技术的最新进展，并为读者提供选择最合适基因表达系统的指南。
