Jang Sanghun, Lim Jaehong, Lee Onseok
Department of Physical Therapy, College of Nursing and Health Science, Gimcheon University, 214, Daehak-ro, Gimcheon City, Gyeongbuk 39528, South Korea.
Pohang Accelerator Laboratory, Industrial Technology Convergence Center, POSTECH, 80, Jigokro-127-beongil, Nam-Gu, Pohang, Gyeongbuk, 37673, South Korea.
Microsc Res Tech. 2017 Nov;80(11):1221-1228. doi: 10.1002/jemt.22920. Epub 2017 Aug 12.
Radiation beam interface contrast X-ray microscopy provides resolution of a few dozen nanometers from fixed whole muscle biopsies, allowing better reconstruction of the microstructure of the muscle than is currently possible with classic histological techniques. Fixed soleus muscle biopsies have been evaluated from the walk-in mouse model using phase-contrast X-ray microscopy, and results presented that corroborate the accuracy of the method used, and its potential for application in physiotherapy and occupational therapy studies. We believe that this method will enhance existing morphometric methods of analysis, leading to accurate reconstruction of other thick specimens that would otherwise require thin sectioning and reconstruction through deconvolution algorithms.
辐射束界面对比度X射线显微镜可对固定的全肌肉活检样本提供几十纳米的分辨率，与目前经典组织学技术相比，能更好地重建肌肉微观结构。使用相衬X射线显微镜对来自步行小鼠模型的固定比目鱼肌活检样本进行了评估，结果证实了所用方法的准确性及其在物理治疗和职业治疗研究中的应用潜力。我们相信，这种方法将改进现有的形态测量分析方法，从而准确重建其他厚样本，否则这些样本需要进行薄切片并通过反卷积算法进行重建。
