Koçak I, Sarac S, Aydogan E, Şentürk E, Akakın D, Koroglu K, Özer Ö F
Department of Otolaringology, Koç University Hospital, Istanbul, Turkey.
Department of Otolaringology, Koç University Hospital, Istanbul, Turkey.
Int J Pediatr Otorhinolaryngol. 2017 Sep;100:247-253. doi: 10.1016/j.ijporl.2017.07.008. Epub 2017 Jul 9.
The purpose of this study is to evaluate the possible protective role of naringenin in gentamicin-induced ototoxicity through an audiological, biochemical and histopathological evaluation.
This study was conducted on 32 adult male rats that were randomized into 4 groups(control, gentamicin, naringenin + gentamicin, and naringenin). Naringenin was given to the rats via oral gavage in a dose of 50 mg/kg/day during the 14 day study period. Gentamicin was given by the intraperitoneal route in a dose of 120 mg/kg/day. Audiological assessment was performed by the distortion product otoacoustic emission (DPOAE) and auditory brainstem response (ABR) measurements, applied to all rats at the beginning of the study, and also on day 14. Biochemical parameters were calculated on day 14 to evaluate the oxidative and antioxidative status. Their cochleae were removed and examined histopathologically, also on day 14. The cochlea of animals were evaluated with the terminal deoxynucleotidyl transferase-mediated dUTPbiotin nick end labeling (TUNEL) method for apoptosis.
On days 14, DPOAE values and ABR thresholds were preserved in group 3(naringenin + gentamicin) when compared with group 2(gentamicin)(p < 0.008). The total oxidant status values and oxidative stress index values were significantly higher in group 2(gentamicin) than in other groups (p < 0.008). The total antioxidant status value was significantly higher in group 3(naringenin + gentamicin) and group 4(naringenin) than in group 2(gentamicin)(p < 0.008). The number of TUNEL positive cells in both the organ of Corti and the stria vascularis were found to be statistically lower in group 3(naringenin + gentamicin) than in group 2(gentamicin)(p < 0.05).
Our study has demonstrated that the ototoxic effect generated by gentamicin could be ameliorated with the concurrent use of naringenin.
本研究旨在通过听力学、生化和组织病理学评估，评价柚皮素在庆大霉素诱导的耳毒性中可能的保护作用。
本研究选取32只成年雄性大鼠，随机分为4组（对照组、庆大霉素组、柚皮素+庆大霉素组和柚皮素组）。在为期14天的研究期间，通过灌胃给予大鼠50mg/kg/天剂量的柚皮素。通过腹腔注射给予大鼠120mg/kg/天剂量的庆大霉素。在研究开始时以及第14天，对所有大鼠进行畸变产物耳声发射（DPOAE）和听性脑干反应（ABR）测量，以进行听力学评估。在第14天计算生化参数，以评估氧化和抗氧化状态。同样在第14天，取出它们的耳蜗进行组织病理学检查。采用末端脱氧核苷酸转移酶介导的dUTP生物素缺口末端标记（TUNEL）法对动物耳蜗进行凋亡评估。
在第14天，与第2组（庆大霉素组）相比，第3组（柚皮素+庆大霉素组）的DPOAE值和ABR阈值得以保留（p<0.008）。第2组（庆大霉素组）的总氧化剂状态值和氧化应激指数值显著高于其他组（p<0.008）。第3组（柚皮素+庆大霉素组）和第4组（柚皮素组）的总抗氧化状态值显著高于第2组（庆大霉素组）（p<0.008）。发现第3组（柚皮素+庆大霉素组）柯蒂氏器和血管纹中TUNEL阳性细胞的数量在统计学上低于第2组（庆大霉素组）（p<0.05）。
我们的研究表明，同时使用柚皮素可减轻庆大霉素产生的耳毒性作用。
