Eroğlu Orkun, Karlıdağ Turgut, Kuloğlu Tuncay, Keleş Erol, Kaygusuz İrfan, Yalçın Şinasi
Department of Otorhinolaryngology, Fırat University School of Medicine, Elazığ, Turkey.
Department of Histology and Embryology, Fırat University School of Medicine, Elazığ, Turkey.
J Int Adv Otol. 2018 Apr;14(1):27-33. doi: 10.5152/iao.2017.3825. Epub 2017 Nov 2.
The aim of this report is to evaluate whether cortexin provides any protective activity against ototoxicity of cisplatin.
The study was performed on 30 healthy adult Wistar Albino rats, and rats were randomly divided into three groups of ten. Group I (Control group) was given intraperitoneal (ip) saline solution 1 mL/day. Group II (Cisplatin group) was given ip cisplatin for 2 days at doses of 10 mg/kg. Group III (Cisplatin + Cortexin group) was given ip cisplatin for 2 days at same doses with ip cortexin 2 mg/day for 7 days. Before and on the fourth day of the study, all subjects underwent auditory brainstem response (ABR) and distortion product otoacoustic emissions (DPOAE) tests. At the end of fourth day, half of the subjects in all three groups were decapitated, and their cochlea were removed for histopathologic examination. On the eighth day, tests of the remaining subjects and histopathological examinations were repeated.
ABR tests on the fourth and eighth days showed elevations in the mean hearing thresholds of Groups II and III compared to Group I (p < 0.05). DPOAE tests revealed a loss in emission values on the fourth and eighth days of the study compared to the baseline in Groups II and III. Comparison of Groups II with III showed that emission loss was higher in Group II at both time points, and the difference was more pronounced on the eighth day. Histopathological findings supported these tests.
Cortexin provide protective activity against cisplatin-induced ototoxicity.
本报告旨在评估皮质素是否对顺铂的耳毒性具有任何保护作用。
对30只健康成年Wistar白化大鼠进行研究，将大鼠随机分为三组，每组10只。第一组（对照组）每天腹腔注射1 mL生理盐水。第二组（顺铂组）以10 mg/kg的剂量腹腔注射顺铂2天。第三组（顺铂+皮质素组）以相同剂量腹腔注射顺铂2天，同时每天腹腔注射2 mg皮质素，共7天。在研究前和研究的第四天，所有受试者均接受听性脑干反应（ABR）和畸变产物耳声发射（DPOAE）测试。在第四天结束时，将三组中一半的受试者断头，取出其耳蜗进行组织病理学检查。在第八天，对其余受试者重复进行测试和组织病理学检查。
与第一组相比，第二组和第三组在第四天和第八天的ABR测试显示平均听力阈值升高（p < 0.05）。DPOAE测试显示，与基线相比，第二组和第三组在研究的第四天和第八天发射值下降。第二组与第三组的比较表明，在两个时间点，第二组的发射损失更高，且在第八天差异更明显。组织病理学结果支持了这些测试。
皮质素对顺铂诱导的耳毒性具有保护作用。
