Petruccioli E, Vanini V, Chiacchio T, Cuzzi G, Cirillo D M, Palmieri F, Ippolito G, Goletti D
Translational Research Unit National Institute for Infectious Disease, L. Spallanzani, Rome, Italy.
Emerging Bacterial Pathogens Unit, Division of Immunology and Infectious Diseases IRCCS, San Raffaele Scientific Institute, Milan, Italy.
Tuberculosis (Edinb). 2017 Sep;106:38-43. doi: 10.1016/j.tube.2017.06.002. Epub 2017 Jun 27.
The QuantiFERON-TB Gold Plus (QFT-Plus) represents the new QuantiFERON-TB Gold In-tube (QFT-GIT) to identify latent tuberculosis infection (LTBI). The main differences is the addition of a new tube containing shorter peptides stimulating CD8 T-cells. Aim of this study is to evaluate the accuracy of QFT-Plus compared with QFT-GIT in a cross sectional study of individuals with or without tuberculosis (TB). We enrolled 179 participants: 19 healthy donors, 58 LTBI, 33 cured TB and 69 active TB. QFT-Plus and QFT-GIT were performed. The two tests showed a substantial agreement. Moreover we found a similar sensitivity in active TB and same specificity in healthy donors. A higher proportion of the LTBI subjects responded to both TB1 and TB2 compared to those with active TB (97% vs 81%). Moreover, a selective response to TB2 was associated with active TB (9%) and with a severe TB disease, suggesting that TB2 stimulation induces a CD8 T-cell response in absence of a CD4-response. In conclusion, QFT-Plus and QFT-GIT assays showed a substantial agreement and similar accuracy for active TB detection. Interestingly, a higher proportion of the LTBI subjects responded concomitantly to TB1 and TB2 compared to those with active TB, whereas a selective TB2 response associated with active TB.
结核感染T细胞检测金标升级版（QFT-Plus）是用于识别潜伏性结核感染（LTBI）的新型结核感染T细胞检测管内检测法（QFT-GIT）。主要区别在于增加了一个新管，其中含有能刺激CD8 T细胞的较短肽段。本研究的目的是在一项针对有或无结核病（TB）个体的横断面研究中，评估QFT-Plus与QFT-GIT相比的准确性。我们招募了179名参与者：19名健康供体、58名LTBI患者、33名已治愈的TB患者和69名活动性TB患者。进行了QFT-Plus和QFT-GIT检测。这两种检测显示出高度一致性。此外，我们发现在活动性TB中两者敏感性相似，在健康供体中特异性相同。与活动性TB患者相比，LTBI受试者中对TB1和TB2均有反应的比例更高（97%对81%）。此外，对TB2的选择性反应与活动性TB（9%）和严重TB疾病相关，这表明在缺乏CD4反应的情况下，TB2刺激可诱导CD8 T细胞反应。总之，QFT-Plus和QFT-GIT检测在检测活动性TB方面显示出高度一致性和相似的准确性。有趣的是，与活动性TB患者相比，LTBI受试者中同时对TB1和TB2有反应的比例更高，而对TB2的选择性反应与活动性TB相关。
